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Advances in enzyme immobilisation

Author: Brady, D ; Jordaan, J Date: 10 Jul 2009 Improvements in current carrier-based immobilisation strategies have been developed using hetero-functionalised supports that enhance the binding efficacy and stability through multipoint attachment. New commercial resins (Sepabeads) exhibit ... Read more
Immobilisation and characterisation of biocatalytic co-factor recycling enzymes, glucose dehydrogenase and NADH oxidase, on aldehyde functional ReSynTM polymer microspheres

Author: Twala, BV ; Sewell, BT ; Jordaan, J Date: Mar 2012 The use of enzymes in industrial applications is limited by their instability, cost and difficulty in their recovery and re-use. Immobilisation is a technique which has been shown to alleviate these limitations in biocatalysis. Here we describe ... Read more
Immobilisation and characterisation of glucose dehydrogenase immobilised on ReSyn: a proprietary polyethylenimine support matrix

Author: Twala, BV ; Sewell, BT ; Jordaan, J Date: Jan 2010 Immobilisation of enzymes is of considerable interest due to the advantages over soluble enzymes, including improved stability and recovery. Glucose Dehydrogenase (GDH) is an important biocatalytic enzyme due to is ability to recycle the ... Read more
Stabilization of Escherichia coli uridine phosphorylase by evolution and immobilization

Author: Visser, Daniel F ; Hennessy F ; Rashamuse J ; Pletschke B ; Brady D Date: Aug 2010 Uridine phosphorylase from Escherichia coli was evolved by iterative saturation mutagenesis. The best mutant showed a temperature optimum of 60C and a half-life of 17.3 h at 60C. The mutant enzyme, as well as a purine nucleoside phosphorylase ... Read more

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Browsing by Subject "Immobilisation"

Browsing by Subject "Immobilisation"

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