Ndlela, Luyanda LSchmidt, S2016-12-052016-12-052016-02Ndlela, L.L. and Schmidt, S. 2016. Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms. SpringerPlus, 5(118), 1-92193-1801http://springerplus.springeropen.com/articles/10.1186/s40064-016-1739-yhttp://hdl.handle.net/10204/8881Copyright: 2016 SpringerOpenThis study assessed faecal matter from three indigenous South African herbivores-zebra, giraffe and impala-as a potential source for hydrolytically active aerobic and facultatively anaerobic bacteria. Herbivore droppings were collected freshly in a local nature reserve in Pietermaritzburg, South Africa. Soil samples adjacent to faecal collection sites and faeces from a domestic herbivore, the Nguni cow, were included as controls. Hydrolase and dehydrogenase activity in faecal matter and soil samples were measured by the fluorescein diacetate and the triphenyltetrazolium chloride assay. Viable counts and counts for amylase, cellulase, esterase and protease producers were established using plate count agar and solid media containing cellulose, skim milk, starch and Tween 80. Zebra droppings produced the highest hydrolase and dehydrogenase activity. Faecal matter of the three indigenous herbivores generally produced higher hydrolytic activity than Nguni cow faeces and soil controls, thereby confirming that these materials are potential targets for hydrolytic enzyme mining.enFaecesFluorescein diacetateGiraffeHydrolasesImpalaNguniTriphenyltetrazolium chlorideZebraArticleNdlela, L., & Schmidt, S. (2016). Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms. http://hdl.handle.net/10204/8881Ndlela, LL, and S Schmidt "Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms." (2016) http://hdl.handle.net/10204/8881Ndlela L, Schmidt S. Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms. 2016; http://hdl.handle.net/10204/8881.TY - Article AU - Ndlela, LL AU - Schmidt, S AB - This study assessed faecal matter from three indigenous South African herbivores-zebra, giraffe and impala-as a potential source for hydrolytically active aerobic and facultatively anaerobic bacteria. Herbivore droppings were collected freshly in a local nature reserve in Pietermaritzburg, South Africa. Soil samples adjacent to faecal collection sites and faeces from a domestic herbivore, the Nguni cow, were included as controls. Hydrolase and dehydrogenase activity in faecal matter and soil samples were measured by the fluorescein diacetate and the triphenyltetrazolium chloride assay. Viable counts and counts for amylase, cellulase, esterase and protease producers were established using plate count agar and solid media containing cellulose, skim milk, starch and Tween 80. Zebra droppings produced the highest hydrolase and dehydrogenase activity. Faecal matter of the three indigenous herbivores generally produced higher hydrolytic activity than Nguni cow faeces and soil controls, thereby confirming that these materials are potential targets for hydrolytic enzyme mining. DA - 2016-02 DB - ResearchSpace DP - CSIR KW - Faeces KW - Fluorescein diacetate KW - Giraffe KW - Hydrolases KW - Impala KW - Nguni KW - Triphenyltetrazolium chloride KW - Zebra LK - https://researchspace.csir.co.za PY - 2016 SM - 2193-1801 T1 - Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms TI - Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms UR - http://hdl.handle.net/10204/8881 ER -