Madala, NETugizimana, FSteenkamp, PA2015-08-192015-08-192014-09Madala, NE, Tugizimana, F and Steenkamp, PA. 2014. Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species. Journal of Analytical Methods in Chemistry, vol. 10, pp 1-82090-8865http://hdl.handle.net/10204/8062Copyright: Hindawi Publishing Corporation 2014.Chlorogenic acids (CGA) have been profiled in the leaves of Momordica balsamina, Momordica charantia, and Momordica foetida. All three species were found to contain the trans and cis isomers of 4-acyl para-coumaroylquinic acid (pCoQA), caffeoylquinic acid (CQA), and feruloylquinic acid (FQA). To the best of our knowledge, this is the first report of pCoQA and FQA and their cis isomers in these Momordica species. These profiles were obtained by a newly developed UPLC-qTOF-MS method based on the in-source collision induced dissociation (ISCID) method optimized to mimic the MS (sup2) andMS(sup3) fragmentation of an ion trap-basedMS. The presence of the cis isomers is believed to be due to high UV exposure of these plants. Furthermore, the absence of the 3-acyl and 5-acyl CGA molecules points to a metabolic mark that is unusual and represents a very interesting biochemical phenotype of these species. Our optimized ISCID method was also shown to be able to distinguish between the geometrical isomers of all three forms of CGA, a phenomenon previously deemed impossible with other common mass spectrometry systems used for CGA analyses.enChlorogenic acidsCGAMomordica balsaminaMomordica charantiaMomordica foetidaArticleMadala, N., Tugizimana, F., & Steenkamp, P. (2014). Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species. http://hdl.handle.net/10204/8062Madala, NE, F Tugizimana, and PA Steenkamp "Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species." (2014) http://hdl.handle.net/10204/8062Madala N, Tugizimana F, Steenkamp P. Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species. 2014; http://hdl.handle.net/10204/8062.TY - Article AU - Madala, NE AU - Tugizimana, F AU - Steenkamp, PA AB - Chlorogenic acids (CGA) have been profiled in the leaves of Momordica balsamina, Momordica charantia, and Momordica foetida. All three species were found to contain the trans and cis isomers of 4-acyl para-coumaroylquinic acid (pCoQA), caffeoylquinic acid (CQA), and feruloylquinic acid (FQA). To the best of our knowledge, this is the first report of pCoQA and FQA and their cis isomers in these Momordica species. These profiles were obtained by a newly developed UPLC-qTOF-MS method based on the in-source collision induced dissociation (ISCID) method optimized to mimic the MS (sup2) andMS(sup3) fragmentation of an ion trap-basedMS. The presence of the cis isomers is believed to be due to high UV exposure of these plants. Furthermore, the absence of the 3-acyl and 5-acyl CGA molecules points to a metabolic mark that is unusual and represents a very interesting biochemical phenotype of these species. Our optimized ISCID method was also shown to be able to distinguish between the geometrical isomers of all three forms of CGA, a phenomenon previously deemed impossible with other common mass spectrometry systems used for CGA analyses. DA - 2014-09 DB - ResearchSpace DP - CSIR KW - Chlorogenic acids KW - CGA KW - Momordica balsamina KW - Momordica charantia KW - Momordica foetida LK - https://researchspace.csir.co.za PY - 2014 SM - 2090-8865 T1 - Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species TI - Development and optimization of an UPLC-QTOF-MS/MS method based on an in-source collision induced dissociation approach for comprehensive discrimination of chlorogenic acids isomers from Momordica plant species UR - http://hdl.handle.net/10204/8062 ER -