Alexandre, Kabamba BGray, ESMufhandu, Hazel TMcMahon, JBChakauya, EO'Keefe, BRChikwamba, Rachel KMorris, L2012-03-232012-03-232012-02Alexandre, KB, Gray, ES, Mufhandu, H, McMahon, JB, Chakauya, E, O'Keefe, BR, Chikwamba, R and Morris, L. The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4+ cells. Virology, vol. 423(2), pp 175-1860042-6822http://www.sciencedirect.com/science/article/pii/S0042682211005587http://ac.els-cdn.com/S0042682211005587/1-s2.0-S0042682211005587-main.pdf?_tid=09606c980c7e0bdd929b9dbf9993890a&acdnat=1332490363_5d94f026a31bbf3915b8bad31a4bb6aahttp://hdl.handle.net/10204/5672Copyright: 2011 Elsevier. This is an ABSTRACT ONLY.It is generally believed that during the sexual transmission of HIV-1, the glycan-specific DC-SIGN receptor binds the virus and mediates its transfer to CD4(+) cells. The lectins griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN) inhibit HIV-1 infection by binding to mannose-rich glycans on gp120. We measured the ability of these lectins to inhibit both the HIV-1 binding to DC-SIGN and the DC-SIGN-mediated HIV-1 infection of CD4(+) cells. While GRFT, CV-N and SVN were moderately inhibitory to DC-SIGN binding, they potently inhibited DC-SIGN-transfer of HIV-1. The introduction of the 234 glycosylation site abolished HIV-1 sensitivity to lectin inhibition of binding to DC-SIGN and virus transfer to susceptible cells. However, the addition of the 295 glycosylation site increased the inhibition of transfer. Our data suggest that GRFT, CV-N and SVN can block two important stages of the sexual transmission of HIV-1, DC-SIGN binding and transfer, supporting their further development as microbicides.enGriffithsinCyanovirin-NScytovirinMannose-rich glycansHIV-1 gp120DC-SIGN receptorArticleAlexandre, K. B., Gray, E., Mufhandu, H. T., McMahon, J., Chakauya, E., O'Keefe, B., ... Morris, L. (2012). The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4+ cells. http://hdl.handle.net/10204/5672Alexandre, Kabamba B, ES Gray, Hazel T Mufhandu, JB McMahon, E Chakauya, BR O'Keefe, Rachel K Chikwamba, and L Morris "The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4+ cells." (2012) http://hdl.handle.net/10204/5672Alexandre KB, Gray E, Mufhandu HT, McMahon J, Chakauya E, O'Keefe B, et al. The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4+ cells. 2012; http://hdl.handle.net/10204/5672.TY - Article AU - Alexandre, Kabamba B AU - Gray, ES AU - Mufhandu, Hazel T AU - McMahon, JB AU - Chakauya, E AU - O'Keefe, BR AU - Chikwamba, Rachel K AU - Morris, L AB - It is generally believed that during the sexual transmission of HIV-1, the glycan-specific DC-SIGN receptor binds the virus and mediates its transfer to CD4(+) cells. The lectins griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN) inhibit HIV-1 infection by binding to mannose-rich glycans on gp120. We measured the ability of these lectins to inhibit both the HIV-1 binding to DC-SIGN and the DC-SIGN-mediated HIV-1 infection of CD4(+) cells. While GRFT, CV-N and SVN were moderately inhibitory to DC-SIGN binding, they potently inhibited DC-SIGN-transfer of HIV-1. The introduction of the 234 glycosylation site abolished HIV-1 sensitivity to lectin inhibition of binding to DC-SIGN and virus transfer to susceptible cells. However, the addition of the 295 glycosylation site increased the inhibition of transfer. Our data suggest that GRFT, CV-N and SVN can block two important stages of the sexual transmission of HIV-1, DC-SIGN binding and transfer, supporting their further development as microbicides. DA - 2012-02 DB - ResearchSpace DP - CSIR KW - Griffithsin KW - Cyanovirin-N KW - Scytovirin KW - Mannose-rich glycans KW - HIV-1 gp120 KW - DC-SIGN receptor LK - https://researchspace.csir.co.za PY - 2012 SM - 0042-6822 T1 - The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4+ cells TI - The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4+ cells UR - http://hdl.handle.net/10204/5672 ER -