Barros, ENelson, SW2011-03-282011-03-282010-06Barros, E and Nelson, SW. 2010. Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission. South African Journal of Science, 106(5/6), pp 40038-2353http://www.scielo.org.za/pdf/sajs/v106n5-6/v106n5-6a18.pdfhttp://hdl.handle.net/10204/4925Copyright: 2010 Academy of Science of South AfricaThe aim of this study was to use conventional breeding to increase the production in maize of the human monoclonal antibody 2G12, known to have potential therapeutic properties in the prevention of HIV-1 transmission. The recombinant antibody, together with a fluorescent marker, was introduced into two South African high-performing maize elite inbred lines by crossing them with a transgenic maize line that had been transformed with the monoclonal antibody 2G12. The effect of breeding to produce high-expressing recombinant hybrid seed was evaluated by comparing 2G12 production in the different breeding lines with the original maize line. 'Good production practice standards' were followed throughout the breeding programme. 'Conventional drug regulations' adapted to plant-made pharmaceuticals were also followed, with the seeds being stored in a 'master seed bank'. The maize hybrid expressed a higher level of the antibody than the recombinant maize elite lines. This plant-derived antibody provides a means of producing a microbicide component that could be used with other HIV-neutralising antibodies as an additional approach to prevent HIV infection.enHIV-1BiopharmingMicrobicideMonoclonal antibodyMaize hybridAIDS2G12HIV vaccine developmentHIV transmissionhigh yielding recombinant maize hybridArticleBarros, E., & Nelson, S. (2010). Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission. http://hdl.handle.net/10204/4925Barros, E, and SW Nelson "Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission." (2010) http://hdl.handle.net/10204/4925Barros E, Nelson S. Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission. 2010; http://hdl.handle.net/10204/4925.TY - Article AU - Barros, E AU - Nelson, SW AB - The aim of this study was to use conventional breeding to increase the production in maize of the human monoclonal antibody 2G12, known to have potential therapeutic properties in the prevention of HIV-1 transmission. The recombinant antibody, together with a fluorescent marker, was introduced into two South African high-performing maize elite inbred lines by crossing them with a transgenic maize line that had been transformed with the monoclonal antibody 2G12. The effect of breeding to produce high-expressing recombinant hybrid seed was evaluated by comparing 2G12 production in the different breeding lines with the original maize line. 'Good production practice standards' were followed throughout the breeding programme. 'Conventional drug regulations' adapted to plant-made pharmaceuticals were also followed, with the seeds being stored in a 'master seed bank'. The maize hybrid expressed a higher level of the antibody than the recombinant maize elite lines. This plant-derived antibody provides a means of producing a microbicide component that could be used with other HIV-neutralising antibodies as an additional approach to prevent HIV infection. DA - 2010-06 DB - ResearchSpace DP - CSIR KW - HIV-1 KW - Biopharming KW - Microbicide KW - Monoclonal antibody KW - Maize hybrid KW - AIDS KW - 2G12 KW - HIV vaccine development KW - HIV transmission KW - high yielding recombinant maize hybrid LK - https://researchspace.csir.co.za PY - 2010 SM - 0038-2353 T1 - Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission TI - Creation of a high yielding recombinant maize hybrid for the production of a microbicide for the prevention of HIV-1 transmission UR - http://hdl.handle.net/10204/4925 ER -