Thobakgale, Setumo LOmbinda-Lemboumba, SaturninMthunzi-Kufa, Patience2020-03-192020-03-192019-02Thobakgale, S.L., Ombinda-Lemboumba, S. & Mthunzi-Kufa, P. 2019. Investigation and calibration of nonessential amino acids using a custom built Raman spectroscopy system. In: Proceedings of SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 1088416, San Francisco, California, United States, 22 February 2019https://doi.org/10.1117/12.2509839https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10885/108850N/Investigation-and-calibration-of-non-essential-amino-acids-using-a/10.1117/12.2509839.full?sessionGUID=02bc9993-ced2-68be-ad49-a28531ab0d3a&webSyncID=6ec7a49d-ab6a-0cba-d059-329ad883c9d9&sessionGUID=02bc9993-ced2-68be-ad49-a28531ab0d3a&SSO=1https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10885/2509839/Investigation-and-calibration-of-non-essential-amino-acids-using-a/10.1117/12.2509839.fullhttp://hdl.handle.net/10204/11348Presented in: Proceedings of SPIE 10885, Optical Diagnostics and Sensing XIX: Toward Point-of-Care Diagnostics, 108850N, San Francisco, California, United States, 20 February 2019. Due to copyright restrictions, the attached PDF file contains the abstract of the full-text item. For access to the full-text item, please consult the publisher's website.Nonessential amino acids (NEAAs) are the building blocks for producing proteins of various functionalities within living systems. For example, from NEAAs, humans produce proteins that are important for cell repair, cell division and synthesis of hormones paramount to maintaining a healthy body. Understanding the chemical properties of NEAAs can therefore allow a thorough investigation of biological processes in the cases of ailing individuals. Raman spectroscopy has become a powerful technique for characterizing various organic compounds such as NEAAs, relying on their unique light scattering properties. In this study we present a custom built Raman Spectroscopy system which was used to investigate and calibrate a group of NEAAs through a comparison of peak area versus concentration. The results show that various regions of interest within the sample mixtures exhibit an increase in signal intensity when the concentration is increased. This is in agreement with current literature on the parameters that effect Raman systems such as concentration. Secondly, it was observed that 0.1 mM of NEAAs was the current detection limit of the system in terms of number of significant peaks produced. As a result, it is important that future work includes incorporating nanomaterials as sample scaffolds for signal enhancement to improve sensitivity. Much of this work is intended towards producing a point-of-care diagnostic tool for analysis of cancer agents.enNonessential amino acidsNEEAsRaman spectroscopy systemsPoint-of-care diagnosticsConference PresentationThobakgale, S. L., Ombinda-Lemboumba, S., & Mthunzi-Kufa, P. (2019). Investigation and calibration of nonessential amino acids using a custom built Raman spectroscopy system. SPIE. http://hdl.handle.net/10204/11348Thobakgale, Setumo L, Saturnin Ombinda-Lemboumba, and Patience Mthunzi-Kufa. "Investigation and calibration of nonessential amino acids using a custom built Raman spectroscopy system." (2019): http://hdl.handle.net/10204/11348Thobakgale SL, Ombinda-Lemboumba S, Mthunzi-Kufa P, Investigation and calibration of nonessential amino acids using a custom built Raman spectroscopy system; SPIE; 2019. http://hdl.handle.net/10204/11348 .TY - Conference Presentation AU - Thobakgale, Setumo L AU - Ombinda-Lemboumba, Saturnin AU - Mthunzi-Kufa, Patience AB - Nonessential amino acids (NEAAs) are the building blocks for producing proteins of various functionalities within living systems. For example, from NEAAs, humans produce proteins that are important for cell repair, cell division and synthesis of hormones paramount to maintaining a healthy body. Understanding the chemical properties of NEAAs can therefore allow a thorough investigation of biological processes in the cases of ailing individuals. Raman spectroscopy has become a powerful technique for characterizing various organic compounds such as NEAAs, relying on their unique light scattering properties. In this study we present a custom built Raman Spectroscopy system which was used to investigate and calibrate a group of NEAAs through a comparison of peak area versus concentration. The results show that various regions of interest within the sample mixtures exhibit an increase in signal intensity when the concentration is increased. This is in agreement with current literature on the parameters that effect Raman systems such as concentration. Secondly, it was observed that 0.1 mM of NEAAs was the current detection limit of the system in terms of number of significant peaks produced. As a result, it is important that future work includes incorporating nanomaterials as sample scaffolds for signal enhancement to improve sensitivity. Much of this work is intended towards producing a point-of-care diagnostic tool for analysis of cancer agents. DA - 2019-02 DB - ResearchSpace DP - CSIR KW - Nonessential amino acids KW - NEEAs KW - Raman spectroscopy systems KW - Point-of-care diagnostics LK - https://researchspace.csir.co.za PY - 2019 T1 - Investigation and calibration of nonessential amino acids using a custom built Raman spectroscopy system TI - Investigation and calibration of nonessential amino acids using a custom built Raman spectroscopy system UR - http://hdl.handle.net/10204/11348 ER -