Lakay, FMBotha, APrior, BA2007-06-292007-06-292007-01Lakay FM, Botha A, Prior BA. 2007. Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils. Journal of Applied Microbiology, 102(1), pp 265-2731364-5072http://hdl.handle.net/10204/756http://www.ncbi.nlm.nih.gov/entrez/query.Three different soil DNA isolation and four purification strategies were compared on different soil samples with variable rates of success. Bead beating extraction gave significantly higher DNA yields than microwave-based and liquid nitrogen grinding DNA extraction methods. The inclusion of soil washing prior to cell lysis decreased the amount of purification steps required. Although these soil types differed, polyvinylpolypyrrolidone (PVPP)-sepharose 2B column elution was sufficient for all three samples, yielding DNA pure enough for successful application in molecular studies. One soil sample retained 80% of the initial DNA after successful purification.enSoil DNA isolationLiquid nitrogenHumic acidSoilArticleLakay, F., Botha, A., & Prior, B. (2007). Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils. http://hdl.handle.net/10204/756Lakay, FM, A Botha, and BA Prior "Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils." (2007) http://hdl.handle.net/10204/756Lakay F, Botha A, Prior B. Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils. 2007; http://hdl.handle.net/10204/756.TY - Article AU - Lakay, FM AU - Botha, A AU - Prior, BA AB - Three different soil DNA isolation and four purification strategies were compared on different soil samples with variable rates of success. Bead beating extraction gave significantly higher DNA yields than microwave-based and liquid nitrogen grinding DNA extraction methods. The inclusion of soil washing prior to cell lysis decreased the amount of purification steps required. Although these soil types differed, polyvinylpolypyrrolidone (PVPP)-sepharose 2B column elution was sufficient for all three samples, yielding DNA pure enough for successful application in molecular studies. One soil sample retained 80% of the initial DNA after successful purification. DA - 2007-01 DB - ResearchSpace DP - CSIR KW - Soil DNA isolation KW - Liquid nitrogen KW - Humic acid KW - Soil LK - https://researchspace.csir.co.za PY - 2007 SM - 1364-5072 T1 - Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils TI - Comparative analysis of environmental DNA extraction and purification methods from different humic acid rich soils UR - http://hdl.handle.net/10204/756 ER -