Van den Berg, NMbobela, PPillay, PLondon, GMorris, LMaharaj, VKhati, M2011-07-082011-07-082011-02Van den Berg, N, Mbobela, P, Pillay, P et al. 2011. HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide. EMBO Global exchange lecture course, Wallenberg Research Centre, Stellenbosch, February 2011http://hdl.handle.net/10204/5091EMBO Global exchange lecture course, Wallenberg Research Centre, Stellenbosch, February 2011A natural compound isolated from extracts of an indigenous plant in the Eastern Cape, South Africa, indicates neutralisation activity against HIV-1 pseudoviruses. This natural compound can potentially be used in a microbicide as an alternative means to prevent the spread of HIV and AIDS. The compound’s neutralisation activity (IC50 0.05-7.2µg/mL) is comparable with that of the entry inhibitor T-20 (IC50 0.05-2.94µg/mL) and the reverse transcriptase inhibitor Tenofovir (IC50 0.09-0.83µg/mL) when screened against the NIH reference panel for HIV-1 subtype C envelope molecular clones. T20 is a more relevant drug for comparative purposes as it is an entry inhibitor and indications on our compound of interest point to it also acting in a similar manner. The mode of action of the compound is, however, yet to be determined. The aim is to develop a microbicide based on an indigenous plant for people infected with HIV-1. The compound will also be screened against other HIV-1 subtypes to test the neutralisation breadth. The authors have shown that the extracts of the plant and the compound are not cytotoxic towards TZM-bl cells when tested in a MTT assay at concentrations up 100 µg/mL. In addition these did not show any neutralisation activity against VSV-G which has a similar glycoprotein to HIV-1 indicating specificity to HIV-1.enNatural compoundsHIV-1HIV-1 pseudovirus neutralisationNeutralisation activityIndigenous plantsConference PresentationVan den Berg, N., Mbobela, P., Pillay, P., London, G., Morris, L., Maharaj, V., & Khati, M. (2011). HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide. CSIR. http://hdl.handle.net/10204/5091Van den Berg, N, P Mbobela, P Pillay, G London, L Morris, V Maharaj, and M Khati. "HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide." (2011): http://hdl.handle.net/10204/5091Van den Berg N, Mbobela P, Pillay P, London G, Morris L, Maharaj V, et al, HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide; CSIR; 2011. http://hdl.handle.net/10204/5091 .TY - Conference Presentation AU - Van den Berg, N AU - Mbobela, P AU - Pillay, P AU - London, G AU - Morris, L AU - Maharaj, V AU - Khati, M AB - A natural compound isolated from extracts of an indigenous plant in the Eastern Cape, South Africa, indicates neutralisation activity against HIV-1 pseudoviruses. This natural compound can potentially be used in a microbicide as an alternative means to prevent the spread of HIV and AIDS. The compound’s neutralisation activity (IC50 0.05-7.2µg/mL) is comparable with that of the entry inhibitor T-20 (IC50 0.05-2.94µg/mL) and the reverse transcriptase inhibitor Tenofovir (IC50 0.09-0.83µg/mL) when screened against the NIH reference panel for HIV-1 subtype C envelope molecular clones. T20 is a more relevant drug for comparative purposes as it is an entry inhibitor and indications on our compound of interest point to it also acting in a similar manner. The mode of action of the compound is, however, yet to be determined. The aim is to develop a microbicide based on an indigenous plant for people infected with HIV-1. The compound will also be screened against other HIV-1 subtypes to test the neutralisation breadth. The authors have shown that the extracts of the plant and the compound are not cytotoxic towards TZM-bl cells when tested in a MTT assay at concentrations up 100 µg/mL. In addition these did not show any neutralisation activity against VSV-G which has a similar glycoprotein to HIV-1 indicating specificity to HIV-1. DA - 2011-02 DB - ResearchSpace DP - CSIR KW - Natural compounds KW - HIV-1 KW - HIV-1 pseudovirus neutralisation KW - Neutralisation activity KW - Indigenous plants LK - https://researchspace.csir.co.za PY - 2011 T1 - HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide TI - HIV-1 pseudovirus neutralisation by a natural compound: a potential microbicide UR - http://hdl.handle.net/10204/5091 ER -