Visser, Daniel FHennessy, FRashamuse, KJLouw, MEBrady, D2010-09-102010-09-102010-03Visser, DF, et al. 2010. Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36 . Extremophiles, Vol. 14(2), pp 185-1921431-0651http://hdl.handle.net/10204/4349Copyright: The Author(s) 2010. This article is published with open access at Springerlink.comA purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 has been cloned and over expressed in E. coli. The purified enzyme had a kcat of 2.03 x 10-9 s-1 and a km of 206 µM on guanosine. The optimal pH range was between 5.7 and 8.4, with an optimal temperature of 70ºC and a half life at 60ºC of 20.8 hours.enNucleoside phosphorylaseBiocatalysisGuanosine5-MethyluridineBacillus haloduransCloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36ArticleVisser, D. F., Hennessy, F., Rashamuse, K., Louw, M., & Brady, D. (2010). Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36. http://hdl.handle.net/10204/4349Visser, Daniel F, F Hennessy, KJ Rashamuse, ME Louw, and D Brady "Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36." (2010) http://hdl.handle.net/10204/4349Visser DF, Hennessy F, Rashamuse K, Louw M, Brady D. Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36. 2010; http://hdl.handle.net/10204/4349.TY - Article AU - Visser, Daniel F AU - Hennessy, F AU - Rashamuse, KJ AU - Louw, ME AU - Brady, D AB - A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 has been cloned and over expressed in E. coli. The purified enzyme had a kcat of 2.03 x 10-9 s-1 and a km of 206 µM on guanosine. The optimal pH range was between 5.7 and 8.4, with an optimal temperature of 70ºC and a half life at 60ºC of 20.8 hours. DA - 2010-03 DB - ResearchSpace DP - CSIR KW - Nucleoside phosphorylase KW - Biocatalysis KW - Guanosine KW - 5-Methyluridine KW - Bacillus halodurans LK - https://researchspace.csir.co.za PY - 2010 SM - 1431-0651 T1 - Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36 TI - Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36 UR - http://hdl.handle.net/10204/4349 ER -