Madala, NESteenkamp, PAPiater, LADubery, IA2013-04-172013-04-172012-07Madala, N.E, Steenkamp, P.A, Piater, L.A and Dubery, I.A. 2012. Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions. Biotechnology Letters, vol. 34(7), pp 1351-13560141-5492http://link.springer.com/article/10.1007/s10529-012-0909-4#http://hdl.handle.net/10204/6671Copyright: 2012 Springer Netherlands. This is an ABSTRACT ONLY. The definitive version is published in Biotechnology Letters, vol.34(7), pp 1351-1356Nicotiana tabacum cell suspensions, 2g wet wt/ml, rapidly took up 1 mM isonitrosoacetophenone (INAP), a plant-derived stress metabolite with anti-oxidative and anti-fungal properties, producing 40-hexopyranosyloxy-30-methoxyisonitrosoacetophenone in 54 %yield over 18 h. Unconverted INAP was at 33 lM. UPLC–MS/MS analyses with MassFragment software were used for metabolite identification. INAP had been hydroxylated at its meta- and para-positions as well as undergoing subsequent methoxylation and glycosylation. INAP is thus recognized by the enzymatic machinery of the phenylpropanoid pathway and is converted to a molecule with a substitution pattern similar to ferulic acid.enBiotransformationIsonitrosoacetophenone2-keto-2-phenyl-acetaldoximeMetabolismNicotiana tabacumSolanaceaeXenobioticsArticleMadala, N., Steenkamp, P., Piater, L., & Dubery, I. (2012). Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions. http://hdl.handle.net/10204/6671Madala, NE, PA Steenkamp, LA Piater, and IA Dubery "Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions." (2012) http://hdl.handle.net/10204/6671Madala N, Steenkamp P, Piater L, Dubery I. Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions. 2012; http://hdl.handle.net/10204/6671.TY - Article AU - Madala, NE AU - Steenkamp, PA AU - Piater, LA AU - Dubery, IA AB - Nicotiana tabacum cell suspensions, 2g wet wt/ml, rapidly took up 1 mM isonitrosoacetophenone (INAP), a plant-derived stress metabolite with anti-oxidative and anti-fungal properties, producing 40-hexopyranosyloxy-30-methoxyisonitrosoacetophenone in 54 %yield over 18 h. Unconverted INAP was at 33 lM. UPLC–MS/MS analyses with MassFragment software were used for metabolite identification. INAP had been hydroxylated at its meta- and para-positions as well as undergoing subsequent methoxylation and glycosylation. INAP is thus recognized by the enzymatic machinery of the phenylpropanoid pathway and is converted to a molecule with a substitution pattern similar to ferulic acid. DA - 2012-07 DB - ResearchSpace DP - CSIR KW - Biotransformation KW - Isonitrosoacetophenone KW - 2-keto-2-phenyl-acetaldoxime KW - Metabolism KW - Nicotiana tabacum KW - Solanaceae KW - Xenobiotics LK - https://researchspace.csir.co.za PY - 2012 SM - 0141-5492 T1 - Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions TI - Biotransformation of isonitrosoacetophenone (2-keto-2-phenyl-acetaldoxime) in tobacco cell suspensions UR - http://hdl.handle.net/10204/6671 ER -