Brady, DDube, NPetersen, RL2009-05-072009-05-072006-09Brady, D, Dube, N and Petersen, RL. 2006. Green chemistry: highly selective biocatalytic hydrolysis of nitrile compounds. South African Journal of Science, Vol.(2006), pp 1-181819-3595http://hdl.handle.net/10204/3354This is the author's version of the work. It is posted here by permission of the Academy of Science of South Africa for your personal use. Not for redistributionThe application of highly substrate-specific catalysts, such as biocatalysts, can reduce the number of synthetic steps required to generate organic compounds. A wide range of bacteria and yeast cultures were enriched on nitriles as the sole source of nitrogen and evaluated for their substrate profiles. The substrates included aliphatic and aromatic nitriles, as well as structurally related amides. Small-scale liquid reactions were monitored using high pressure liquid chromatography and gas chromatography, combined with mass spectroscopy. The microbial biocatalysts demonstrated a wide variation of activities between genera, within genera, and even within species. The range of substrates transformed and the inter- and intra-species differences in specificity of the individual biocatalysts, suggests that it is possible to provide multiple catalytic or bioremediation agents for the fine chemicals industryenGreen chemistryBiocatalytic hydrolysisNitrile compoundsHydrolytic enzymesBacteriaYeastNitrilesArticleBrady, D., Dube, N., & Petersen, R. (2006). Green chemistry: highly selective biocatalytic hydrolysis of nitrile compounds. http://hdl.handle.net/10204/3354Brady, D, N Dube, and RL Petersen "Green chemistry: highly selective biocatalytic hydrolysis of nitrile compounds." (2006) http://hdl.handle.net/10204/3354Brady D, Dube N, Petersen R. Green chemistry: highly selective biocatalytic hydrolysis of nitrile compounds. 2006; http://hdl.handle.net/10204/3354.TY - Article AU - Brady, D AU - Dube, N AU - Petersen, RL AB - The application of highly substrate-specific catalysts, such as biocatalysts, can reduce the number of synthetic steps required to generate organic compounds. A wide range of bacteria and yeast cultures were enriched on nitriles as the sole source of nitrogen and evaluated for their substrate profiles. The substrates included aliphatic and aromatic nitriles, as well as structurally related amides. Small-scale liquid reactions were monitored using high pressure liquid chromatography and gas chromatography, combined with mass spectroscopy. The microbial biocatalysts demonstrated a wide variation of activities between genera, within genera, and even within species. The range of substrates transformed and the inter- and intra-species differences in specificity of the individual biocatalysts, suggests that it is possible to provide multiple catalytic or bioremediation agents for the fine chemicals industry DA - 2006-09 DB - ResearchSpace DP - CSIR KW - Green chemistry KW - Biocatalytic hydrolysis KW - Nitrile compounds KW - Hydrolytic enzymes KW - Bacteria KW - Yeast KW - Nitriles LK - https://researchspace.csir.co.za PY - 2006 SM - 1819-3595 T1 - Green chemistry: highly selective biocatalytic hydrolysis of nitrile compounds TI - Green chemistry: highly selective biocatalytic hydrolysis of nitrile compounds UR - http://hdl.handle.net/10204/3354 ER -