Fanucchi, StephanieShibayama, YBurd, SWeinberg, MSMhlanga, Musa2014-02-132014-02-132013-10Fanucchi, S, Shibayama, Y, Burd, S, Weinberg, M.S and Mhlanga, M. 2013. Chromosomal contact permits transcription between coregulated genes. Cell, vol. 155(3), pp 606-6200092-8674http://www.sciencedirect.com/science/article/pii/S0092867413012257http://hdl.handle.net/10204/7207Copyright: 2013 Elsevier. This is an ABSTRACT ONLY. The definitive version is published in Cell, vol. 155(3), pp 606-620Transcription of coregulated genes occurs in the context of long-range chromosomal contacts that form multigene complexes. Such contacts and transcription are lost in knockout studies of transcription factors and structural chromatin proteins. To ask whether chromosomal contacts are required for cotranscription in multigene complexes, we devised a strategy using TALENs to cleave and disrupt gene loops in a well-characterized multigene complex. Monitoring this disruption using RNA FISH and immunofluorescence microscopy revealed that perturbing the site of contact had a direct effect on transcription of other interacting genes. Unexpectedly, this effect on cotranscription was hierarchical, with dominant and subordinate members of the multigene complex engaged in both intra- and interchromosomal contact. This observation reveals the profound influence of these chromosomal contacts on the transcription of coregulated genes in a multigene complex.enCoregulated genesLong-range chromosomal contactsMultigenesArticleFanucchi, S., Shibayama, Y., Burd, S., Weinberg, M., & Mhlanga, M. (2013). Chromosomal contact permits transcription between coregulated genes. http://hdl.handle.net/10204/7207Fanucchi, Stephanie, Y Shibayama, S Burd, MS Weinberg, and Musa Mhlanga "Chromosomal contact permits transcription between coregulated genes." (2013) http://hdl.handle.net/10204/7207Fanucchi S, Shibayama Y, Burd S, Weinberg M, Mhlanga M. Chromosomal contact permits transcription between coregulated genes. 2013; http://hdl.handle.net/10204/7207.TY - Article AU - Fanucchi, Stephanie AU - Shibayama, Y AU - Burd, S AU - Weinberg, MS AU - Mhlanga, Musa AB - Transcription of coregulated genes occurs in the context of long-range chromosomal contacts that form multigene complexes. Such contacts and transcription are lost in knockout studies of transcription factors and structural chromatin proteins. To ask whether chromosomal contacts are required for cotranscription in multigene complexes, we devised a strategy using TALENs to cleave and disrupt gene loops in a well-characterized multigene complex. Monitoring this disruption using RNA FISH and immunofluorescence microscopy revealed that perturbing the site of contact had a direct effect on transcription of other interacting genes. Unexpectedly, this effect on cotranscription was hierarchical, with dominant and subordinate members of the multigene complex engaged in both intra- and interchromosomal contact. This observation reveals the profound influence of these chromosomal contacts on the transcription of coregulated genes in a multigene complex. DA - 2013-10 DB - ResearchSpace DP - CSIR KW - Coregulated genes KW - Long-range chromosomal contacts KW - Multigenes LK - https://researchspace.csir.co.za PY - 2013 SM - 0092-8674 T1 - Chromosomal contact permits transcription between coregulated genes TI - Chromosomal contact permits transcription between coregulated genes UR - http://hdl.handle.net/10204/7207 ER -