Van Brummelen, ACBecker, JVWMancama, Dalubuhle THoppe, H2010-03-032010-03-032010-01Van Brummelen AC, Becker JVW et al. 2010. Establishing malaria parasite transfection technology in South Africa. 22nd South African Society for Biochemistry and Molecular Biology Congress. Bloemfontein, South Africa, 18-20 January 2010, pp 2http://hdl.handle.net/10204/397122nd South African Society for Biochemistry and Molecular Biology Congress. Bloemfontein, South Africa, 18-20 January 2010In order to establish malaria parasite transfection technology in South Africa, firefly luciferase and green fluorescent protein (GFP) reporter constructs were prepared. In attempt to simplify these constructs, a var intron (PFC0005w), previously reported to have bidirectional promoter activity, was utilized to drive expression through two genes (i.e. the antibiotic-resistance gene, human dhfr and the reporter gene) in a head-to-head orientation. In addition, protocols were adjusted by including DNA packaging reagents to improve uptake into the parasite and by using shaking instead of stationary parasite cultures to improve the parasite proliferation and selection rate. Successfully transfected parasites were selected by the antifolate WR99210.enPlasmodium falciparumAnti-malarial drugAnti-malarial drug resistanceTransfectionMalariaParasite transfection technologyBiochemistryMolecular biologyConference PresentationVan Brummelen, A., Becker, J., Mancama, D. T., & Hoppe, H. (2010). Establishing malaria parasite transfection technology in South Africa. http://hdl.handle.net/10204/3971Van Brummelen, AC, JVW Becker, Dalubuhle T Mancama, and H Hoppe. "Establishing malaria parasite transfection technology in South Africa." (2010): http://hdl.handle.net/10204/3971Van Brummelen A, Becker J, Mancama DT, Hoppe H, Establishing malaria parasite transfection technology in South Africa; 2010. http://hdl.handle.net/10204/3971 .TY - Conference Presentation AU - Van Brummelen, AC AU - Becker, JVW AU - Mancama, Dalubuhle T AU - Hoppe, H AB - In order to establish malaria parasite transfection technology in South Africa, firefly luciferase and green fluorescent protein (GFP) reporter constructs were prepared. In attempt to simplify these constructs, a var intron (PFC0005w), previously reported to have bidirectional promoter activity, was utilized to drive expression through two genes (i.e. the antibiotic-resistance gene, human dhfr and the reporter gene) in a head-to-head orientation. In addition, protocols were adjusted by including DNA packaging reagents to improve uptake into the parasite and by using shaking instead of stationary parasite cultures to improve the parasite proliferation and selection rate. Successfully transfected parasites were selected by the antifolate WR99210. DA - 2010-01 DB - ResearchSpace DP - CSIR KW - Plasmodium falciparum KW - Anti-malarial drug KW - Anti-malarial drug resistance KW - Transfection KW - Malaria KW - Parasite transfection technology KW - Biochemistry KW - Molecular biology LK - https://researchspace.csir.co.za PY - 2010 T1 - Establishing malaria parasite transfection technology in South Africa TI - Establishing malaria parasite transfection technology in South Africa UR - http://hdl.handle.net/10204/3971 ER -