De Souza Gil, ERezende, SGRibeiro Júnior, EJMBarcelos, HTScalize, PSSantiago, MFQuintino, MPSomerset, VS2015-03-122015-03-122014De Souza Gil, E, Rezende, S.G, Ribeiro Júnior, E.J.M, Barcelos, H.T, Scalize, P.S, Santiago, M.F, Quintino, M.P and Somerset, V.S. 2014. Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters. In: Sensing in Electroanalysis, University Press Centre, Pardubice, Czech Republic, vol.8, pp 227-240978-80-7395-782-7http://dspace.upce.cz/bitstream/10195/58400/1/SouzaGilE_DevelopmentOfLaccase_2014.pdfhttp://hdl.handle.net/10204/7936Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website.Laccase is a poliphenoloxidase enzyme that catalyzes the oxidation of phenolic compounds in the corresponding quinones. The current obtained in this redox process can be used for quantitative analysis. In this work, a carbon paste biosensor modified gluteraldehyde functionalized silica and an enzymatic extract of the Pycnoporus sanguineus fungi as a lacase source is proposed for phenol determination. The effect of carbon paste and electrolyte composition, pH from 3.0 to 8.0, start potential from 0.55 to 0.25 mV, scan rate from 5 to 25 mV (sups-1) and potential pulse amplitude from 10 to 60 mV on the differential pulse voltammetric response was investigated. A linear correlation of r² = 0.9946 was obtained for the phenol content (catechol) in the concentration range from 50 to 500 nmol L(sup-1), with a detection limit of 30 nmol L(sup-1). This biosensor was used for the determination of different kinds of phenolic compounds, presenting a better response for catechol.enBiosensorLaccasePhenolic micropollutantsCatecholPycnoporus sanguineus fungiFunctionalised silicaBook ChapterDe Souza Gil, E., Rezende, S., Ribeiro Júnior, E., Barcelos, H., Scalize, P., Santiago, M., ... Somerset, V. (2014). Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters., <i>Workflow;14109</i> University Press Centre. http://hdl.handle.net/10204/7936De Souza Gil, E, SG Rezende, EJM Ribeiro Júnior, HT Barcelos, PS Scalize, MF Santiago, MP Quintino, and VS Somerset. "Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters" In <i>WORKFLOW;14109</i>, n.p.: University Press Centre. 2014. http://hdl.handle.net/10204/7936.De Souza Gil E, Rezende S, Ribeiro Júnior E, Barcelos H, Scalize P, Santiago M, et al. Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters.. Workflow;14109. [place unknown]: University Press Centre; 2014. [cited yyyy month dd]. http://hdl.handle.net/10204/7936.TY - Book Chapter AU - De Souza Gil, E AU - Rezende, SG AU - Ribeiro Júnior, EJM AU - Barcelos, HT AU - Scalize, PS AU - Santiago, MF AU - Quintino, MP AU - Somerset, VS AB - Laccase is a poliphenoloxidase enzyme that catalyzes the oxidation of phenolic compounds in the corresponding quinones. The current obtained in this redox process can be used for quantitative analysis. In this work, a carbon paste biosensor modified gluteraldehyde functionalized silica and an enzymatic extract of the Pycnoporus sanguineus fungi as a lacase source is proposed for phenol determination. The effect of carbon paste and electrolyte composition, pH from 3.0 to 8.0, start potential from 0.55 to 0.25 mV, scan rate from 5 to 25 mV (sups-1) and potential pulse amplitude from 10 to 60 mV on the differential pulse voltammetric response was investigated. A linear correlation of r² = 0.9946 was obtained for the phenol content (catechol) in the concentration range from 50 to 500 nmol L(sup-1), with a detection limit of 30 nmol L(sup-1). This biosensor was used for the determination of different kinds of phenolic compounds, presenting a better response for catechol. DA - 2014 DB - ResearchSpace DP - CSIR KW - Biosensor KW - Laccase KW - Phenolic micropollutants KW - Catechol KW - Pycnoporus sanguineus fungi KW - Functionalised silica LK - https://researchspace.csir.co.za PY - 2014 SM - 978-80-7395-782-7 T1 - Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters TI - Development of a Laccase biosensor for determination of phenolic micropollutants in surface waters UR - http://hdl.handle.net/10204/7936 ER -