Ngamsom, BFourie, LTarn, MDKumar, SMoodley, KLand, KPamme, N2017-04-102017-04-102016-10Ngamsom, B., Fourie, L., Tarn, M.D., Kumar, S., Moodley, K., Land, K. and Pamme, N. 2016. Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis. In: Proceedings of the 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), 9-13 October 2016, Dublin, Ireland978-0-9798064-9-0http://hdl.handle.net/10204/8990Proceedings of the 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), 9-13 October 2016, Dublin, IrelandThe present investigation reports isolation and detection of E. coli O157:H7 employing a simple and portable microfluidic device based on immiscible filtration assisted by surface tension (IFAST) and adenosine triphosphate (ATP) bioluminescence assay. The device demonstrated ca. 90% E. coli isolation with linear responses of bioluminescence signals from isolated cells at 6 – 600 CFU mL-1, suggesting great potential for point-of-need pathogenic bacteria detection for water analysis.enE. coli detectionWater analysisImmiscible filtration assisted by surface tensionIFASTAdenosine triphosphateATPMicrofluidicsMicrosystemPathogen detection in waterRapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysisConference PresentationNgamsom, B., Fourie, L., Tarn, M., Kumar, S., Moodley, K., Land, K., & Pamme, N. (2016). Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis. http://hdl.handle.net/10204/8990Ngamsom, B, L Fourie, MD Tarn, S Kumar, K Moodley, K Land, and N Pamme. "Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis." (2016): http://hdl.handle.net/10204/8990Ngamsom B, Fourie L, Tarn M, Kumar S, Moodley K, Land K, et al, Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis; 2016. http://hdl.handle.net/10204/8990 .TY - Conference Presentation AU - Ngamsom, B AU - Fourie, L AU - Tarn, MD AU - Kumar, S AU - Moodley, K AU - Land, K AU - Pamme, N AB - The present investigation reports isolation and detection of E. coli O157:H7 employing a simple and portable microfluidic device based on immiscible filtration assisted by surface tension (IFAST) and adenosine triphosphate (ATP) bioluminescence assay. The device demonstrated ca. 90% E. coli isolation with linear responses of bioluminescence signals from isolated cells at 6 – 600 CFU mL-1, suggesting great potential for point-of-need pathogenic bacteria detection for water analysis. DA - 2016-10 DB - ResearchSpace DP - CSIR KW - E. coli detection KW - Water analysis KW - Immiscible filtration assisted by surface tension KW - IFAST KW - Adenosine triphosphate KW - ATP KW - Microfluidics KW - Microsystem KW - Pathogen detection in water LK - https://researchspace.csir.co.za PY - 2016 SM - 978-0-9798064-9-0 T1 - Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis TI - Rapid detection of E. Coli O157:H7 by IFAST and ATP bioluminescence assay for water analysis UR - http://hdl.handle.net/10204/8990 ER -