Stander, BAvan Vollenstee, FAKallmeyer, KPotgieter, MJoubert, ASwanepoel, AndriKotzé-Jacobs, LaraMoolman, SPepper, MS2018-08-152018-08-152018-07Stander, B.A. et al. 2018. An in vitro and in vivo study on the properties of hollow polycaprolactone cell-delivery particles. PLoS ONE, vol. 13(7): e01982481932-6203https://doi.org/10.1371/journal.pone.0198248http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0198248http://hdl.handle.net/10204/10362Open access article published in PLoS ONE, vol. 13(7): e0198248The field of dermal fillers is evolving rapidly and numerous products are currently on the market. Biodegradable polymers such as polycaprolactone (PCL) have been found to be compatible with several body tissues, and this makes them an ideal material for dermal filling purposes. Hollow PCL spheres were developed by the Council for Scientific and Industrial Research (CSIR) to serve both as an anchor point and a "tissue harbour" for cells. Particles were tested for cytotoxicity and cell adherence using mouse embryo fibroblasts (MEF). MEFs adhered to the particles and no significant toxic effects were observed based on morphology, cell growth, cell viability and cell cycle analysis, suggesting that the particles are suitable candidates for cell delivery systems in an in vivo setting. The objective of providing a "tissue harbour" was however not realized, as cells did not preferentially migrate into the ported particles. In vivo studies were conducted in BALB/c mice into whom particles were introduced at the level of the hypodermis. Mice injected with PCL particles (ported and nonported; with or without MEFs) showed evidence of local inflammation and increased adipogenesis at the site of injection, as well as a systemic inflammatory response. These effects were also observed in mice that received apparently inert (polystyrene) particles. Ported PCL particles can therefore act as a cell delivery system and through their ability to induce adipogenesis, may also serve as a dermal bulking agent.enpolycaprolactone cell-delivery particlesdermal fillersbiodegradable polymersMEFsmouse embryo fibroblastsdermal bulking agentArticleStander, B., van Vollenstee, F., Kallmeyer, K., Potgieter, M., Joubert, A., Swanepoel, A., ... Pepper, M. (2018). An in vitro and in vivo study on the properties of hollow polycaprolactone cell-delivery particles. http://hdl.handle.net/10204/10362Stander, BA, FA van Vollenstee, K Kallmeyer, M Potgieter, A Joubert, Andri Swanepoel, Lara Kotzé-Jacobs, S Moolman, and MS Pepper "An in vitro and in vivo study on the properties of hollow polycaprolactone cell-delivery particles." (2018) http://hdl.handle.net/10204/10362Stander B, van Vollenstee F, Kallmeyer K, Potgieter M, Joubert A, Swanepoel A, et al. An in vitro and in vivo study on the properties of hollow polycaprolactone cell-delivery particles. 2018; http://hdl.handle.net/10204/10362.TY - Article AU - Stander, BA AU - van Vollenstee, FA AU - Kallmeyer, K AU - Potgieter, M AU - Joubert, A AU - Swanepoel, Andri AU - Kotzé-Jacobs, Lara AU - Moolman, S AU - Pepper, MS AB - The field of dermal fillers is evolving rapidly and numerous products are currently on the market. Biodegradable polymers such as polycaprolactone (PCL) have been found to be compatible with several body tissues, and this makes them an ideal material for dermal filling purposes. Hollow PCL spheres were developed by the Council for Scientific and Industrial Research (CSIR) to serve both as an anchor point and a "tissue harbour" for cells. Particles were tested for cytotoxicity and cell adherence using mouse embryo fibroblasts (MEF). MEFs adhered to the particles and no significant toxic effects were observed based on morphology, cell growth, cell viability and cell cycle analysis, suggesting that the particles are suitable candidates for cell delivery systems in an in vivo setting. The objective of providing a "tissue harbour" was however not realized, as cells did not preferentially migrate into the ported particles. In vivo studies were conducted in BALB/c mice into whom particles were introduced at the level of the hypodermis. Mice injected with PCL particles (ported and nonported; with or without MEFs) showed evidence of local inflammation and increased adipogenesis at the site of injection, as well as a systemic inflammatory response. These effects were also observed in mice that received apparently inert (polystyrene) particles. Ported PCL particles can therefore act as a cell delivery system and through their ability to induce adipogenesis, may also serve as a dermal bulking agent. DA - 2018-07 DB - ResearchSpace DP - CSIR KW - polycaprolactone cell-delivery particles KW - dermal fillers KW - biodegradable polymers KW - MEFs KW - mouse embryo fibroblasts KW - dermal bulking agent LK - https://researchspace.csir.co.za PY - 2018 SM - 1932-6203 T1 - An in vitro and in vivo study on the properties of hollow polycaprolactone cell-delivery particles TI - An in vitro and in vivo study on the properties of hollow polycaprolactone cell-delivery particles UR - http://hdl.handle.net/10204/10362 ER -