Oldach, KHMorgenstern, ARother, SGirgi, MO'Kennedy, Maretha MLorz, H2007-02-062007-06-072007-02-062007-06-072001-07Oldach, KH, et al. 2001. Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench. Plant Cell Reports, vol. 20(5), pp 416-4210721-7714http://hdl.handle.net/10204/1540http://hdl.handle.net/10204/1540The authors report an in vitro culture system that provides reliable, highly efficient regeneration from immature embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and sorghum [Sorghum bicolor (L.) Moench]. Immature embryos were isolated 10-20 days after pollination and cultured on various L3 media. The influence of different parameters during the callus induction phase was examined with respect to the regeneration rate: (1) the concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and various cytokinins; (2) the addition of AgNO3; (3) the use of maltose or sucrose as a carbon source. Modifications in the phytohormones alone resulted in the regeneration of fertile sorghum plants at high efficiency. Significant increases in the regeneration rates of pearl millet genotypes were achieved by the combination of sucrose as a carbon source and silver nitrate as a potential ethylene inhibitor.248276 bytesapplication/pdfenCopyright: 2001 Springer-VerlagSomatic embryogenesisSorghum bicolor MoenchPearl millet immature embryosPennisetum glaucum (L.) R. BrPlant sciencesArticleOldach, K., Morgenstern, A., Rother, S., Girgi, M., O'Kennedy, M. M., & Lorz, H. (2001). Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench. http://hdl.handle.net/10204/1540Oldach, KH, A Morgenstern, S Rother, M Girgi, Maretha M O'Kennedy, and H Lorz "Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench." (2001) http://hdl.handle.net/10204/1540Oldach K, Morgenstern A, Rother S, Girgi M, O'Kennedy MM, Lorz H. Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench. 2001; http://hdl.handle.net/10204/1540.TY - Article AU - Oldach, KH AU - Morgenstern, A AU - Rother, S AU - Girgi, M AU - O'Kennedy, Maretha M AU - Lorz, H AB - The authors report an in vitro culture system that provides reliable, highly efficient regeneration from immature embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and sorghum [Sorghum bicolor (L.) Moench]. Immature embryos were isolated 10-20 days after pollination and cultured on various L3 media. The influence of different parameters during the callus induction phase was examined with respect to the regeneration rate: (1) the concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and various cytokinins; (2) the addition of AgNO3; (3) the use of maltose or sucrose as a carbon source. Modifications in the phytohormones alone resulted in the regeneration of fertile sorghum plants at high efficiency. Significant increases in the regeneration rates of pearl millet genotypes were achieved by the combination of sucrose as a carbon source and silver nitrate as a potential ethylene inhibitor. DA - 2001-07 DB - ResearchSpace DP - CSIR KW - Somatic embryogenesis KW - Sorghum bicolor Moench KW - Pearl millet immature embryos KW - Pennisetum glaucum (L.) R. Br KW - Plant sciences LK - https://researchspace.csir.co.za PY - 2001 SM - 0721-7714 T1 - Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench TI - Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennisetum glaucum (L.) R. Br.] and Sorghum bicolor (L.) Moench UR - http://hdl.handle.net/10204/1540 ER -