Bode, MLBuddoo, SRMinnaar, SHDu Plessis, CA2009-05-182009-05-182008-08Bode, ML, Buddoo, SR, Minnaar, SH and Du Plessis, CA. 2008. Extraction, isolation and NMR data of the tetraether lipid calditoglycerocaldarchaeol (GDNT) from Sulfolobus metallicus harvested from a bioleaching reactor. Chemistry and Physics of Lipids, Vol (2008) pp 1-200009-3084http://hdl.handle.net/10204/3382Author Posting. Copyright Elsevier, 2008. This is the author's version of the work. It is posted here by permission of Elsevier for personal use, not for redistributionThe successful extraction and isolation of the hydrolysed tetraether lipid calditoglycerocaldarchaeol (GDNT) from Sulfolobus etallicus, a key thermophilic bioleaching archaeon, is described. The archaeal biomasswas recovered directly from a thermophilic (68 C) bioleaching tank reactor used to extract nickel from a pentlandite mineral concentrate. The initial Soxhlet extraction method employed was scaled to a bench-scale extraction procedure suitable for the preparation of gram-scale quantities of GDNT. The GDNT so obtained was analysed by 1D- and 2D-NMR techniques, providing the first complete 13C and 2D-NMR data-set for GDNT, including that for the intact underivatised calditol moiety. The study demonstrates the feasibility of recovering high-quality GNDT from thermophilic archaeal-mediated bioleaching reactors. The recovery of these lipids at relatively low cost, as a by-product from bioleaching reactors used in the metals processing industry, has important implications for future tetraether lipid availability and costsenSulfolobus metallicusTetraether lipidGlycerol dialkyl glycerol tetraether2D-NMRBioleachingGlycerol dialkyl nonitol tetraetherGDNTBioleaching reactorArticleBode, M., Buddoo, S., Minnaar, S., & Du Plessis, C. (2008). Extraction, isolation and NMR data of the tetraether lipid calditoglycerocaldarchaeol (GDNT) from Sulfolobus metallicus harvested from a bioleaching reactor. http://hdl.handle.net/10204/3382Bode, ML, SR Buddoo, SH Minnaar, and CA Du Plessis "Extraction, isolation and NMR data of the tetraether lipid calditoglycerocaldarchaeol (GDNT) from Sulfolobus metallicus harvested from a bioleaching reactor." (2008) http://hdl.handle.net/10204/3382Bode M, Buddoo S, Minnaar S, Du Plessis C. Extraction, isolation and NMR data of the tetraether lipid calditoglycerocaldarchaeol (GDNT) from Sulfolobus metallicus harvested from a bioleaching reactor. 2008; http://hdl.handle.net/10204/3382.TY - Article AU - Bode, ML AU - Buddoo, SR AU - Minnaar, SH AU - Du Plessis, CA AB - The successful extraction and isolation of the hydrolysed tetraether lipid calditoglycerocaldarchaeol (GDNT) from Sulfolobus etallicus, a key thermophilic bioleaching archaeon, is described. The archaeal biomasswas recovered directly from a thermophilic (68 C) bioleaching tank reactor used to extract nickel from a pentlandite mineral concentrate. The initial Soxhlet extraction method employed was scaled to a bench-scale extraction procedure suitable for the preparation of gram-scale quantities of GDNT. The GDNT so obtained was analysed by 1D- and 2D-NMR techniques, providing the first complete 13C and 2D-NMR data-set for GDNT, including that for the intact underivatised calditol moiety. The study demonstrates the feasibility of recovering high-quality GNDT from thermophilic archaeal-mediated bioleaching reactors. The recovery of these lipids at relatively low cost, as a by-product from bioleaching reactors used in the metals processing industry, has important implications for future tetraether lipid availability and costs DA - 2008-08 DB - ResearchSpace DP - CSIR KW - Sulfolobus metallicus KW - Tetraether lipid KW - Glycerol dialkyl glycerol tetraether KW - 2D-NMR KW - Bioleaching KW - Glycerol dialkyl nonitol tetraether KW - GDNT KW - Bioleaching reactor LK - https://researchspace.csir.co.za PY - 2008 SM - 0009-3084 T1 - Extraction, isolation and NMR data of the tetraether lipid calditoglycerocaldarchaeol (GDNT) from Sulfolobus metallicus harvested from a bioleaching reactor TI - Extraction, isolation and NMR data of the tetraether lipid calditoglycerocaldarchaeol (GDNT) from Sulfolobus metallicus harvested from a bioleaching reactor UR - http://hdl.handle.net/10204/3382 ER -