Khoza, BSChimuka, LMukwevho, ESteenkamp, PAMadala, NE2014-12-022014-12-022014-10Khoza, B.S, Chimuka, L, Mukwevho, E, Steenkamp, P.A, and Madala, N.E. 2014. The effect of temperature on pressurised hot water extraction of pharmacologically important metabolites as analysed by UPLC-qTOF-MS and PCA. Evidence-Based Complementary and Alternative Medicine, vol. 2014 (9147590),pp 1-91741-427Xhttp://www.hindawi.com/journals/ecam/2014/914759/http://hdl.handle.net/10204/7797Copyright: 2014 Hindawi Publishing Corporation. This is an OA journal. The journal authorizes the publication of the information herewith contained. Published in Evidence-Based Complementary and Alternative Medicine, vol. 2014(914759), pp 1-9Metabolite extraction methods have been shown to be a critical consideration for pharmacometabolomics studies and, as such, optimization and development of new extraction methods are crucial. In the current study, an organic solvent-free method, namely, pressurised hot water extraction (PHWE), was used to extract pharmacologically important metabolites from dried Moringa oleifera leaves. Here, the temperature of the extraction solvent (pure water) was altered while keeping other factors constant using a homemade PHWE system. Samples extracted at different temperatures (50, 100, and 150 degrees C) were assayed for antioxidant activities and the effect of the temperature on the extraction process was evaluated. The samples were further analysed by mass spectrometry to elucidate their metabolite compositions. Principal component analysis (PCA) evaluation of the UPLC-MS data showed distinctive differential metabolite patterns. Here, temperature changes during PHWE were shown to affect the levels of metabolites with known pharmacological activities, such as chlorogenic acids and flavonoids. Our overall findings suggest that, if not well optimised, the extraction temperature could compromise the “pharmacological potency” of the extracts. The use of MS in combination with PCA was furthermore shown to be an excellent approach to evaluate the quality and content of pharmacologically important extracts.enMetabolite extraction methodsMoringa oleifera leavesPressurised hot water extractionPHWEArticleKhoza, B., Chimuka, L., Mukwevho, E., Steenkamp, P., & Madala, N. (2014). The effect of temperature on pressurised hot water extraction of pharmacologically important metabolites as analysed by UPLC-qTOF-MS and PCA. http://hdl.handle.net/10204/7797Khoza, BS, L Chimuka, E Mukwevho, PA Steenkamp, and NE Madala "The effect of temperature on pressurised hot water extraction of pharmacologically important metabolites as analysed by UPLC-qTOF-MS and PCA." (2014) http://hdl.handle.net/10204/7797Khoza B, Chimuka L, Mukwevho E, Steenkamp P, Madala N. The effect of temperature on pressurised hot water extraction of pharmacologically important metabolites as analysed by UPLC-qTOF-MS and PCA. 2014; http://hdl.handle.net/10204/7797.TY - Article AU - Khoza, BS AU - Chimuka, L AU - Mukwevho, E AU - Steenkamp, PA AU - Madala, NE AB - Metabolite extraction methods have been shown to be a critical consideration for pharmacometabolomics studies and, as such, optimization and development of new extraction methods are crucial. In the current study, an organic solvent-free method, namely, pressurised hot water extraction (PHWE), was used to extract pharmacologically important metabolites from dried Moringa oleifera leaves. Here, the temperature of the extraction solvent (pure water) was altered while keeping other factors constant using a homemade PHWE system. Samples extracted at different temperatures (50, 100, and 150 degrees C) were assayed for antioxidant activities and the effect of the temperature on the extraction process was evaluated. The samples were further analysed by mass spectrometry to elucidate their metabolite compositions. Principal component analysis (PCA) evaluation of the UPLC-MS data showed distinctive differential metabolite patterns. Here, temperature changes during PHWE were shown to affect the levels of metabolites with known pharmacological activities, such as chlorogenic acids and flavonoids. Our overall findings suggest that, if not well optimised, the extraction temperature could compromise the “pharmacological potency” of the extracts. The use of MS in combination with PCA was furthermore shown to be an excellent approach to evaluate the quality and content of pharmacologically important extracts. DA - 2014-10 DB - ResearchSpace DP - CSIR KW - Metabolite extraction methods KW - Moringa oleifera leaves KW - Pressurised hot water extraction KW - PHWE LK - https://researchspace.csir.co.za PY - 2014 SM - 1741-427X T1 - The effect of temperature on pressurised hot water extraction of pharmacologically important metabolites as analysed by UPLC-qTOF-MS and PCA TI - The effect of temperature on pressurised hot water extraction of pharmacologically important metabolites as analysed by UPLC-qTOF-MS and PCA UR - http://hdl.handle.net/10204/7797 ER -