High-yielding cascade enzymatic synthesis of 5-methyluridine using a novel combination of nucleoside phosphorylases

Visser, Daniel FRashamuse, KJHennessy, FGordon, GERVan Zyl, PJMathiba, KBode, MLBrady, DLouw, ME2010-09-302010-09-302010-07Visser, DF, Rashamuse, KJ, Hennessy, F et al. 2010. High-yielding cascade enzymatic synthesis of 5-methyluridine using a novel combination of nucleoside phosphorylases. Biocatalysis and Biotransformation, Vol. 28(4), pp 245–2531024-2422http://informahealthcare.com/doi/abs/10.3109/10242422.2010.493210?journalCode=babhttp://hdl.handle.net/10204/4428Copyright: 2010 Informa Healthcare. This is the author's pre print version. The definitive version is published in Biocatalysis and Biotransformation, Vol. 28(4), pp 245–253A novel combination of Bacillus halodurans purine nucleoside phosphorylase (BhPNP1) and Escherichia coli uridine phosphorylase (EcUP) has been applied to a dual-enzyme, sequential, biocatalytic one-pot synthesis of 5-methyluridine from guanosine and thymine. A 5-methyluridine yield of 79% on guanosine was achieved in a reaction slurry at a 53 mM (1.5% w/w) guanosine concentration. 5-Methyluridine is an intermediate in synthetic routes to thymidine and the antiretroviral drugs zidovudine and stavudine.enPurine nucleoside phosphorylaseUridine phosphorylaseBiocatalysis5-methyluridineFermentationTransglycosylationHigh-yielding cascade enzymatic synthesis of 5-methyluridine using a novel combination of nucleoside phosphorylasesArticleVisser, D. F., Rashamuse, K., Hennessy, F., Gordon, G., Van Zyl, P., Mathiba, K., ... Louw, M. (2010). High-yielding cascade enzymatic synthesis of 5-methyluridine using a novel combination of nucleoside phosphorylases. http://hdl.handle.net/10204/4428Visser, Daniel F, KJ Rashamuse, F Hennessy, GER Gordon, PJ Van Zyl, K Mathiba, ML Bode, D Brady, and ME Louw "High-yielding cascade enzymatic synthesis of 5-methyluridine using a novel combination of nucleoside phosphorylases." (2010) http://hdl.handle.net/10204/4428Visser DF, Rashamuse K, Hennessy F, Gordon G, Van Zyl P, Mathiba K, et al. High-yielding cascade enzymatic synthesis of 5-methyluridine using a novel combination of nucleoside phosphorylases. 2010; http://hdl.handle.net/10204/4428.TY - Article AU - Visser, Daniel F AU - Rashamuse, KJ AU - Hennessy, F AU - Gordon, GER AU - Van Zyl, PJ AU - Mathiba, K AU - Bode, ML AU - Brady, D AU - Louw, ME AB - A novel combination of Bacillus halodurans purine nucleoside phosphorylase (BhPNP1) and Escherichia coli uridine phosphorylase (EcUP) has been applied to a dual-enzyme, sequential, biocatalytic one-pot synthesis of 5-methyluridine from guanosine and thymine. A 5-methyluridine yield of 79% on guanosine was achieved in a reaction slurry at a 53 mM (1.5% w/w) guanosine concentration. 5-Methyluridine is an intermediate in synthetic routes to thymidine and the antiretroviral drugs zidovudine and stavudine. DA - 2010-07 DB - ResearchSpace DP - CSIR KW - Purine nucleoside phosphorylase KW - Uridine phosphorylase KW - Biocatalysis KW - 5-methyluridine KW - Fermentation KW - Transglycosylation LK - https://researchspace.csir.co.za PY - 2010 SM - 1024-2422 T1 - High-yielding cascade enzymatic synthesis of 5-methyluridine using a novel combination of nucleoside phosphorylases TI - High-yielding cascade enzymatic synthesis of 5-methyluridine using a novel combination of nucleoside phosphorylases UR - http://hdl.handle.net/10204/4428 ER -