O'Kennedy, Maretha MCrampton, BGLorito, MChakauya, EBreese, WABurger, JTBotha, FC2011-07-252011-07-252011-05O’Kennedy, MM, Crampton BG, Lorito M, et al. 2011. Expression of a ß-1, 3-glucanase from a biocontrol fungus in transgenic pearl millet. SA Journal of Botany, Vol. 77, pp. 335-3450254-6299http://scholar.sun.ac.za/handle/10019.1/14691http://hdl.handle.net/10204/5115Copyright: 2011 Elsevier. This is a post print version of the work. The definitive version is published in SA Journal of Botany, Vol. 77, pp. 335-345Sclerospora graminicola is an Oomycete (heterotrophic Stramenopiles), fungal-like obligate phytopathogen, the causal agent of downy mildew in pearl millet (Pennisetum glaucum [L.] R. Br.), and a major constraint in the production of this cereal crop. In this study a hydrolytic enzyme, ß-1,3-glucanase, from the biocontrol fungus Trichoderma atroviride (gluc78) was introduced into the genome of a pearl millet breeding line, 842B, by particle bombardment. Constructs were prepared containing the gluc78 gene, encoding the 78 kDa ß-1,3-glucanase protein, driven either by a constitutive ubiquitin promoter or the wound inducible potato proteinase inhibitor IIK gene promoter (pin2). The positive selectable marker gene, manA, encoding mannose-6-phosphate isomerase (phosphomannose isomerase) driven by the ubiquitin promoter, was used for co-transformation. Transgenic plants were obtained harbouring the manA selectable marker gene and the antifungal gene gluc78 driven either by the ubiquitin or pin2 promoter. Full constructs or minimal transgene expression cassettes containing the genes of interest were successfully introduced into the genome of pearl millet. Progeny of stably transformed plants, harbouring the gluc78 transgene which is driven by the pin2 promoter and followed by the rice Act1 intron sequences, were subjected to pathogenicity trials. Apart from one isolated event reducing incidence of S. graminicola infection by 58%, transgenic pearl millet showed no resistance to this phytopathogen. The event conferring decreased susceptibility to S. graminicola had high levels of the glucanase transcript especially in transgenic plants showing severe downy mildew infection.enFungal resistanceß-1,3-glucanaseSclerospora graminicolaTransgenic pearl milletFungusArticleO'Kennedy, M., Crampton, B., Lorito, M., Chakauya, E., Breese, W., Burger, J., & Botha, F. (2011). Expression of a ß-1,3-glucanase from a biocontrol fungus in transgenic pearl millet. http://hdl.handle.net/10204/5115O'Kennedy, MM, BG Crampton, M Lorito, E Chakauya, WA Breese, JT Burger, and FC Botha "Expression of a ß-1,3-glucanase from a biocontrol fungus in transgenic pearl millet." (2011) http://hdl.handle.net/10204/5115O'Kennedy M, Crampton B, Lorito M, Chakauya E, Breese W, Burger J, et al. Expression of a ß-1,3-glucanase from a biocontrol fungus in transgenic pearl millet. 2011; http://hdl.handle.net/10204/5115.TY - Article AU - O'Kennedy, MM AU - Crampton, BG AU - Lorito, M AU - Chakauya, E AU - Breese, WA AU - Burger, JT AU - Botha, FC AB - Sclerospora graminicola is an Oomycete (heterotrophic Stramenopiles), fungal-like obligate phytopathogen, the causal agent of downy mildew in pearl millet (Pennisetum glaucum [L.] R. Br.), and a major constraint in the production of this cereal crop. In this study a hydrolytic enzyme, ß-1,3-glucanase, from the biocontrol fungus Trichoderma atroviride (gluc78) was introduced into the genome of a pearl millet breeding line, 842B, by particle bombardment. Constructs were prepared containing the gluc78 gene, encoding the 78 kDa ß-1,3-glucanase protein, driven either by a constitutive ubiquitin promoter or the wound inducible potato proteinase inhibitor IIK gene promoter (pin2). The positive selectable marker gene, manA, encoding mannose-6-phosphate isomerase (phosphomannose isomerase) driven by the ubiquitin promoter, was used for co-transformation. Transgenic plants were obtained harbouring the manA selectable marker gene and the antifungal gene gluc78 driven either by the ubiquitin or pin2 promoter. Full constructs or minimal transgene expression cassettes containing the genes of interest were successfully introduced into the genome of pearl millet. Progeny of stably transformed plants, harbouring the gluc78 transgene which is driven by the pin2 promoter and followed by the rice Act1 intron sequences, were subjected to pathogenicity trials. Apart from one isolated event reducing incidence of S. graminicola infection by 58%, transgenic pearl millet showed no resistance to this phytopathogen. The event conferring decreased susceptibility to S. graminicola had high levels of the glucanase transcript especially in transgenic plants showing severe downy mildew infection. DA - 2011-05 DB - ResearchSpace DP - CSIR KW - Fungal resistance KW - ß-1,3-glucanase KW - Sclerospora graminicola KW - Transgenic pearl millet KW - Fungus LK - https://researchspace.csir.co.za PY - 2011 SM - 0254-6299 T1 - Expression of a ß-1,3-glucanase from a biocontrol fungus in transgenic pearl millet TI - Expression of a ß-1,3-glucanase from a biocontrol fungus in transgenic pearl millet UR - http://hdl.handle.net/10204/5115 ER -