Mohanrasu, KGuru Raj Rao, RDinesh, GHZhang, KSiva Prakash, GSong, DMuniyasamy, SudhakarPugazhendhi, AJeyakanthan, AArun, A2021-04-052021-04-052020-07Mohanrasu, K., Guru Raj Rao, R., Dinesh, G., Zhang, K., Siva Prakash, G., Song, D., Muniyasamy, S. & Pugazhendhi, A. et al. 2020. Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium. <i>Fuel, 271.</i> http://hdl.handle.net/10204/119300016-23611873-7153http://hdl.handle.net/10204/11930Polyhydroxybutyrate (PHB) accumulating Bacillus megaterium was isolated from marine water. To increase the PHB productivity by Bacillus megaterium, steps were taken to evaluate the effects of carbon sources (arabinose, glucose, glycerol, lactose, lactic acid, mannitol, sodium acetate, starch and sucrose at a level of 20 g/L), nitrogen sources (ammonium chloride, ammonium sulphate, glycine, potassium nitrate, protease peptone and urea at a level of 2 g/L) and different pH. A maximum yield of 2.74 g/L of PHB was achieved for glucose as the carbon source and ammonium sulphate as the nitrogen source at pH 7. The optimized conditions were further used for batch fermentation throughout 72 h. Significantly maximum PHB of 5.61 g/L was obtained in a laboratory scale bioreactor at 64 h. The extracted polymer was compared with the authentic PHB and was confirmed to be PHB using FTIR, 1H NMR, DSC and TGA analysis, respectively.AbstractenBioreactorBacillus megateriumNuclear magnetic resonanceNMRPolyhydroxybutyratesPHAsThermo gravimetric analysisTGAArticleMohanrasu, K., Guru Raj Rao, R., Dinesh, G., Zhang, K., Siva Prakash, G., Song, D., ... Arun, A. (2020). Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium. <i>Fuel, 271</i>, http://hdl.handle.net/10204/11930Mohanrasu, K, R Guru Raj Rao, GH Dinesh, K Zhang, G Siva Prakash, D Song, Sudhakar Muniyasamy, A Pugazhendhi, A Jeyakanthan, and A Arun "Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium." <i>Fuel, 271</i> (2020) http://hdl.handle.net/10204/11930Mohanrasu K, Guru Raj Rao R, Dinesh G, Zhang K, Siva Prakash G, Song D, et al. Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium. Fuel, 271. 2020; http://hdl.handle.net/10204/11930.TY - Article AU - Mohanrasu, K AU - Guru Raj Rao, R AU - Dinesh, GH AU - Zhang, K AU - Siva Prakash, G AU - Song, D AU - Muniyasamy, Sudhakar AU - Pugazhendhi, A AU - Jeyakanthan, A AU - Arun, A AB - Polyhydroxybutyrate (PHB) accumulating Bacillus megaterium was isolated from marine water. To increase the PHB productivity by Bacillus megaterium, steps were taken to evaluate the effects of carbon sources (arabinose, glucose, glycerol, lactose, lactic acid, mannitol, sodium acetate, starch and sucrose at a level of 20 g/L), nitrogen sources (ammonium chloride, ammonium sulphate, glycine, potassium nitrate, protease peptone and urea at a level of 2 g/L) and different pH. A maximum yield of 2.74 g/L of PHB was achieved for glucose as the carbon source and ammonium sulphate as the nitrogen source at pH 7. The optimized conditions were further used for batch fermentation throughout 72 h. Significantly maximum PHB of 5.61 g/L was obtained in a laboratory scale bioreactor at 64 h. The extracted polymer was compared with the authentic PHB and was confirmed to be PHB using FTIR, 1H NMR, DSC and TGA analysis, respectively. DA - 2020-07 DB - ResearchSpace DP - CSIR J1 - Fuel, 271 KW - Bioreactor KW - Bacillus megaterium KW - Nuclear magnetic resonance KW - NMR KW - Polyhydroxybutyrates KW - PHAs KW - Thermo gravimetric analysis KW - TGA LK - https://researchspace.csir.co.za PY - 2020 SM - 0016-2361 SM - 1873-7153 T1 - Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium TI - Optimization of media components and culture conditions for polyhydroxyalkanoates production by Bacillus megaterium UR - http://hdl.handle.net/10204/11930 ER -24119