Gcebe, NRutten, VPMGGey van Pittius, NNaicker, BrendonMichel, AL2018-08-232018-08-232018-03Gcebe, N. et al. 2018. Mycobacterium komaniense sp. nov., a rapidly growing nontuberculous Mycobacterium species detected in South Africa. International Journal of Systematic and Evolutionary Microbiology, vol. 68(5): 1526-15321466-50341466-5026http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002707DOI 10.1099/ijsem.0.002707http://hdl.handle.net/10204/10385Copyright: 2018 IUMS. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website. The article can be accessed at: http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002707Some species of non-tuberculous mycobacteria (NTM) have been reported to be opportunistic pathogens of animals and humans. Recently there has been an upsurge in the number of cases of NTM infections, such that some NTM species are now recognized as pathogens of humans and animals. From a veterinary point of view, the major significance of NTM is the cross–reactive immune response they elicit against Mycobacterium bovis antigens, leading to misdiagnosis of bovine tuberculosis. Four NTM isolates were detected from a bovine nasal swab, soil and water, during an NTM survey in South Africa. These were all found using 16S rRNA gene sequence analysis to be closely related to Mycobacterium moriokaense. The isolates were further characterised by sequence analysis of the partial fragments of hsp65, rpoB and sodA. The genome of the type strain was also elucidated. Gene (16S rRNA, hsp65, rpoB and sodA) and protein sequence data analysis of 6 kDa early secretory antigenic target (ESAT 6) and 10 kDa culture filtrate protein (CFP-10) revealed that these isolates belong to a unique Mycobacterium species. Differences in phenotypic and biochemical traits between the isolates and closely related species further supported that these isolates belong to novel Mycobacterium species. We proposed the name Mycobacterium komaniense sp. nov. for this new species. The type strain is GPK 1020T (=CIP 110823T=ATCC BAA-2758).enMycobacterium komaniense sp.novnon tuberculous MycobacteriumESAT 6CFP-10ArticleGcebe, N., Rutten, V., Gey van Pittius, N., Naicker, B., & Michel, A. (2018). Mycobacterium komaniense sp. nov., a rapidly growing nontuberculous Mycobacterium species detected in South Africa. http://hdl.handle.net/10204/10385Gcebe, N, VPMG Rutten, N Gey van Pittius, Brendon Naicker, and AL Michel "Mycobacterium komaniense sp. nov., a rapidly growing nontuberculous Mycobacterium species detected in South Africa." (2018) http://hdl.handle.net/10204/10385Gcebe N, Rutten V, Gey van Pittius N, Naicker B, Michel A. Mycobacterium komaniense sp. nov., a rapidly growing nontuberculous Mycobacterium species detected in South Africa. 2018; http://hdl.handle.net/10204/10385.TY - Article AU - Gcebe, N AU - Rutten, VPMG AU - Gey van Pittius, N AU - Naicker, Brendon AU - Michel, AL AB - Some species of non-tuberculous mycobacteria (NTM) have been reported to be opportunistic pathogens of animals and humans. Recently there has been an upsurge in the number of cases of NTM infections, such that some NTM species are now recognized as pathogens of humans and animals. From a veterinary point of view, the major significance of NTM is the cross–reactive immune response they elicit against Mycobacterium bovis antigens, leading to misdiagnosis of bovine tuberculosis. Four NTM isolates were detected from a bovine nasal swab, soil and water, during an NTM survey in South Africa. These were all found using 16S rRNA gene sequence analysis to be closely related to Mycobacterium moriokaense. The isolates were further characterised by sequence analysis of the partial fragments of hsp65, rpoB and sodA. The genome of the type strain was also elucidated. Gene (16S rRNA, hsp65, rpoB and sodA) and protein sequence data analysis of 6 kDa early secretory antigenic target (ESAT 6) and 10 kDa culture filtrate protein (CFP-10) revealed that these isolates belong to a unique Mycobacterium species. Differences in phenotypic and biochemical traits between the isolates and closely related species further supported that these isolates belong to novel Mycobacterium species. We proposed the name Mycobacterium komaniense sp. nov. for this new species. The type strain is GPK 1020T (=CIP 110823T=ATCC BAA-2758). DA - 2018-03 DB - ResearchSpace DP - CSIR KW - Mycobacterium komaniense sp.nov KW - non tuberculous Mycobacterium KW - ESAT 6 KW - CFP-10 LK - https://researchspace.csir.co.za PY - 2018 SM - 1466-5034 SM - 1466-5026 T1 - Mycobacterium komaniense sp. nov., a rapidly growing nontuberculous Mycobacterium species detected in South Africa TI - Mycobacterium komaniense sp. nov., a rapidly growing nontuberculous Mycobacterium species detected in South Africa UR - http://hdl.handle.net/10204/10385 ER -