Mhlongo, MITugizimana, FPiater, LASteenkamp, Paul AMadala, NEDubery, IA2018-07-092018-07-092017-01Mhlongo, M.I. et al. 2017. Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells. Biochemical and Biophysical Research Communications, vol. 482(4): 1498-15030006-291X1090-2104https://www.sciencedirect.com/science/article/pii/S0006291X16321076doi.org/10.1016/j.bbrc.2016.12.063http://hdl.handle.net/10204/10291Copyright: 2017 Elsevier. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website.To counteract biotic stress factors, plants employ multilayered defense mechanisms responsive to pathogen-derived elicitor molecules, and regulated by different phytohormones and signaling molecules. Here, lipopolysaccharide (LPS), a microbe-associated molecular pattern (MAMP) molecule, was used to induce defense responses in Nicotiana tabacum cell suspensions. Intracellular metabolites were extracted with methanol and analyzed using a liquid chromatography-mass spectrometry (UHPLC-qTOF-MS/MS) platform. The generated data were processed and examined with multivariate and univariate statistical tools. The results show time-dependent dynamic changes and accumulation of glycosylated signaling molecules, specifically those of azelaic acid, salicylic acid and methyl-salicylate as contributors to the altered metabolomic state in LPS-treated cells.enAzelaic acidLipopolysaccharideMetabolomicsPhytohormonesSalicylic acidArticleMhlongo, M., Tugizimana, F., Piater, L., Steenkamp, P. A., Madala, N., & Dubery, I. (2017). Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells. http://hdl.handle.net/10204/10291Mhlongo, MI, F Tugizimana, LA Piater, Paul A Steenkamp, NE Madala, and IA Dubery "Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells." (2017) http://hdl.handle.net/10204/10291Mhlongo M, Tugizimana F, Piater L, Steenkamp PA, Madala N, Dubery I. Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells. 2017; http://hdl.handle.net/10204/10291.TY - Article AU - Mhlongo, MI AU - Tugizimana, F AU - Piater, LA AU - Steenkamp, Paul A AU - Madala, NE AU - Dubery, IA AB - To counteract biotic stress factors, plants employ multilayered defense mechanisms responsive to pathogen-derived elicitor molecules, and regulated by different phytohormones and signaling molecules. Here, lipopolysaccharide (LPS), a microbe-associated molecular pattern (MAMP) molecule, was used to induce defense responses in Nicotiana tabacum cell suspensions. Intracellular metabolites were extracted with methanol and analyzed using a liquid chromatography-mass spectrometry (UHPLC-qTOF-MS/MS) platform. The generated data were processed and examined with multivariate and univariate statistical tools. The results show time-dependent dynamic changes and accumulation of glycosylated signaling molecules, specifically those of azelaic acid, salicylic acid and methyl-salicylate as contributors to the altered metabolomic state in LPS-treated cells. DA - 2017-01 DB - ResearchSpace DP - CSIR KW - Azelaic acid KW - Lipopolysaccharide KW - Metabolomics KW - Phytohormones KW - Salicylic acid LK - https://researchspace.csir.co.za PY - 2017 SM - 0006-291X SM - 1090-2104 T1 - Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells TI - Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells UR - http://hdl.handle.net/10204/10291 ER -