Mabaso, Matsilele AWithey, Daniel JTwala, B2019-01-162019-01-162018Mabaso, M.A., Withey, D.J. and Twala, B. 2018. Spot detection methods in fluorescence microscopy imaging: A review. Image Analysis & Stereology, vol. 37(3): 173-1901580-3139https://www.ias-iss.org/ojs/IAS/article/view/1690http://hdl.handle.net/10204/10606Article published in Image Analysis & Stereology, vol. 37(3): 173-190. This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.Fluorescence microscopy imaging has become one of the essential tools used by biologists to visualize and study intracellular particles within a cell. Studying these particles is a long-term research effort in the field of microscopy image analysis, consisting of discovering the relationship between the dynamics of particles and their functions. However, biologists are faced with challenges such as the counting and tracking of these intracellular particles. To overcome the issues faced by biologists, tools which can extract the location and motion of these particles are essential. One of the most important steps in these analyses is to accurately detect particle positions in an image, termed spot detection. The detection of spots in microscopy imaging is seen as a critical step for further quantitative analysis. However, the evaluation of these microscopic images is mainly conducted manually, with automated methods becoming popular. This work presents some advances in fluorescence microscopy image analysis, focusing on the detection methods needed for quantifying the location of these spots. We review several existing detection methods in microscopy imaging, along with existing synthetic benchmark datasets and evaluation metrics.enFluorescence microscopyMicroscopy image analysisSpot detectionSupervisedUnsupervisedArticleMabaso, M. A., Withey, D. J., & Twala, B. (2018). Spot detection methods in fluorescence microscopy imaging: A review. http://hdl.handle.net/10204/10606Mabaso, Matsilele A, Daniel J Withey, and B Twala "Spot detection methods in fluorescence microscopy imaging: A review." (2018) http://hdl.handle.net/10204/10606Mabaso MA, Withey DJ, Twala B. Spot detection methods in fluorescence microscopy imaging: A review. 2018; http://hdl.handle.net/10204/10606.TY - Article AU - Mabaso, Matsilele A AU - Withey, Daniel J AU - Twala, B AB - Fluorescence microscopy imaging has become one of the essential tools used by biologists to visualize and study intracellular particles within a cell. Studying these particles is a long-term research effort in the field of microscopy image analysis, consisting of discovering the relationship between the dynamics of particles and their functions. However, biologists are faced with challenges such as the counting and tracking of these intracellular particles. To overcome the issues faced by biologists, tools which can extract the location and motion of these particles are essential. One of the most important steps in these analyses is to accurately detect particle positions in an image, termed spot detection. The detection of spots in microscopy imaging is seen as a critical step for further quantitative analysis. However, the evaluation of these microscopic images is mainly conducted manually, with automated methods becoming popular. This work presents some advances in fluorescence microscopy image analysis, focusing on the detection methods needed for quantifying the location of these spots. We review several existing detection methods in microscopy imaging, along with existing synthetic benchmark datasets and evaluation metrics. DA - 2018 DB - ResearchSpace DP - CSIR KW - Fluorescence microscopy KW - Microscopy image analysis KW - Spot detection KW - Supervised KW - Unsupervised LK - https://researchspace.csir.co.za PY - 2018 SM - 1580-3139 T1 - Spot detection methods in fluorescence microscopy imaging: A review TI - Spot detection methods in fluorescence microscopy imaging: A review UR - http://hdl.handle.net/10204/10606 ER -