Chhiba, Varsha PBode, MMathiba, KKwezi, LusisizweBrady, D2013-01-292013-01-292012-04Chhiba, V, Bode, M, Mathiba, K, Kwezi, W and Brady, D. 2012. Enantioselective biocatalytic hydrolysis of ß-aminonitriles to ß-amino-amides using Rhodococcus rhodochrous ATCC BAA-870. Journal of Molecular Catalysis B: Enzymatic, Vol. 76, pp 68-7413811177http://www.sciencedirect.com/science/article/pii/S1381117711003225http://hdl.handle.net/10204/6485Copyright: 2012 Elsevier. This is the post print version of the work. The definitive version is published in Journal of Molecular Catalysis B: Enzymatic, Vol. 76, pp 68-74A range of ß-aminonitriles (3-amino-3-phenylpropanenitrile and derivatives) were synthesised by reaction of various benzonitriles with acetonitrile and subsequent reduction of the resulting acrylonitrile products. These compounds were hydrolysed to the corresponding amides using the nitrile biocatalytic activity of Rhodococccus rhodochrousATCCBAA-870. Results showed that the nitrile hydratase enzyme was enantioselective for these compounds, in particular 3-amino-3-p-tolylpropanenitrile and 3-amino-3-(4- methoxyphenyl)propanenitrile and the corresponding amides (up to 85% in one case). The reactions were performed at pH 9.0 after initial attempts at pH 7.0 were unsuccessful, most likely as a result of protonation of the 3-amino group at the lower pH.enNitrile hydrataseAmidaseß-aminonitrileBiocatalysisBiotransformationRhodococcusKinetic resolutionArticleChhiba, V. P., Bode, M., Mathiba, K., Kwezi, L., & Brady, D. (2012). Enantioselective biocatalytic hydrolysis of ß-aminonitriles to ß-amino-amides using Rhodococcus rhodochrous ATCC BAA-870. http://hdl.handle.net/10204/6485Chhiba, Varsha P, M Bode, K Mathiba, Lusisizwe Kwezi, and D Brady "Enantioselective biocatalytic hydrolysis of ß-aminonitriles to ß-amino-amides using Rhodococcus rhodochrous ATCC BAA-870." (2012) http://hdl.handle.net/10204/6485Chhiba VP, Bode M, Mathiba K, Kwezi L, Brady D. Enantioselective biocatalytic hydrolysis of ß-aminonitriles to ß-amino-amides using Rhodococcus rhodochrous ATCC BAA-870. 2012; http://hdl.handle.net/10204/6485.TY - Article AU - Chhiba, Varsha P AU - Bode, M AU - Mathiba, K AU - Kwezi, Lusisizwe AU - Brady, D AB - A range of ß-aminonitriles (3-amino-3-phenylpropanenitrile and derivatives) were synthesised by reaction of various benzonitriles with acetonitrile and subsequent reduction of the resulting acrylonitrile products. These compounds were hydrolysed to the corresponding amides using the nitrile biocatalytic activity of Rhodococccus rhodochrousATCCBAA-870. Results showed that the nitrile hydratase enzyme was enantioselective for these compounds, in particular 3-amino-3-p-tolylpropanenitrile and 3-amino-3-(4- methoxyphenyl)propanenitrile and the corresponding amides (up to 85% in one case). The reactions were performed at pH 9.0 after initial attempts at pH 7.0 were unsuccessful, most likely as a result of protonation of the 3-amino group at the lower pH. DA - 2012-04 DB - ResearchSpace DP - CSIR KW - Nitrile hydratase KW - Amidase KW - ß-aminonitrile KW - Biocatalysis KW - Biotransformation KW - Rhodococcus KW - Kinetic resolution LK - https://researchspace.csir.co.za PY - 2012 SM - 13811177 T1 - Enantioselective biocatalytic hydrolysis of ß-aminonitriles to ß-amino-amides using Rhodococcus rhodochrous ATCC BAA-870 TI - Enantioselective biocatalytic hydrolysis of ß-aminonitriles to ß-amino-amides using Rhodococcus rhodochrous ATCC BAA-870 UR - http://hdl.handle.net/10204/6485 ER -