Hamese, SaltielMugwanda, KanganwiroTakundwa, Mutsa MPrinsloo, EThimiri Govindaraj, Deepak B2024-01-112024-01-112023-12Hamese, S., Mugwanda, K., Takundwa, M.M., Prinsloo, E. & Thimiri Govindaraj, D.B. 2023. Recent advances in genome annotation and synthetic biology for the development of microbial chassis. <i>Journal of Genetic Engineering and Biotechnology, 21(156).</i> http://hdl.handle.net/10204/135051687-157X2090-5920https://doi.org/10.1186/s43141-023-00598-3http://hdl.handle.net/10204/13505This article provides an overview of microbial host selection, synthetic biology, genome annotation, metabolic modeling, and computational methods for predicting gene essentiality for developing a microbial chassis. This article focuses on lactic acid bacteria (LAB) as a microbial chassis and strategies for genome annotation of the LAB genome. As a case study, Lactococcus lactis is chosen based on its well-established therapeutic applications such as probiotics and oral vaccine development. In this article, we have delineated the strategies for genome annotations of lactic acid bacteria. These strategies also provide insights into streamlining genome reduction without compromising the functionality of the chassis and the potential for minimal genome chassis development. These insights underscore the potential for the development of efficient and sustainable synthetic biology systems using streamlined microbial chassis with minimal genomes.FulltextenSynthetic biologyGenetic engineeringGenome annotationMetabolic modelingArticleHamese, S., Mugwanda, K., Takundwa, M. M., Prinsloo, E., & Thimiri Govindaraj, D. B. (2023). Recent advances in genome annotation and synthetic biology for the development of microbial chassis. <i>Journal of Genetic Engineering and Biotechnology, 21(156)</i>, http://hdl.handle.net/10204/13505Hamese, Saltiel, Kanganwiro Mugwanda, Mutsa M Takundwa, E Prinsloo, and Deepak B Thimiri Govindaraj "Recent advances in genome annotation and synthetic biology for the development of microbial chassis." <i>Journal of Genetic Engineering and Biotechnology, 21(156)</i> (2023) http://hdl.handle.net/10204/13505Hamese S, Mugwanda K, Takundwa MM, Prinsloo E, Thimiri Govindaraj DB. Recent advances in genome annotation and synthetic biology for the development of microbial chassis. Journal of Genetic Engineering and Biotechnology, 21(156). 2023; http://hdl.handle.net/10204/13505.TY - Article AU - Hamese, Saltiel AU - Mugwanda, Kanganwiro AU - Takundwa, Mutsa M AU - Prinsloo, E AU - Thimiri Govindaraj, Deepak B AB - This article provides an overview of microbial host selection, synthetic biology, genome annotation, metabolic modeling, and computational methods for predicting gene essentiality for developing a microbial chassis. This article focuses on lactic acid bacteria (LAB) as a microbial chassis and strategies for genome annotation of the LAB genome. As a case study, Lactococcus lactis is chosen based on its well-established therapeutic applications such as probiotics and oral vaccine development. In this article, we have delineated the strategies for genome annotations of lactic acid bacteria. These strategies also provide insights into streamlining genome reduction without compromising the functionality of the chassis and the potential for minimal genome chassis development. These insights underscore the potential for the development of efficient and sustainable synthetic biology systems using streamlined microbial chassis with minimal genomes. DA - 2023-12 DB - ResearchSpace DP - CSIR J1 - Journal of Genetic Engineering and Biotechnology, 21(156) KW - Synthetic biology KW - Genetic engineering KW - Genome annotation KW - Metabolic modeling LK - https://researchspace.csir.co.za PY - 2023 SM - 1687-157X SM - 2090-5920 T1 - Recent advances in genome annotation and synthetic biology for the development of microbial chassis TI - Recent advances in genome annotation and synthetic biology for the development of microbial chassis UR - http://hdl.handle.net/10204/13505 ER -27420