Saasa, ValentineBeukes, MLemmer, YolandyMwakikunga, Bonex W2020-07-272020-07-272019-12Saasa, V., Beukes, M., Lemmer, Y. & Mwakikunga, B.W. 2019. Blood ketone bodies and breath acetone analysis and their correlations in Type 2 Diabetes Mellitus. Diagnostics, vol 9(4): 224. doi: 10.3390/diagnostics90402242075-4418doi: 10.3390/diagnostics9040224https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963753/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963753/pdf/diagnostics-09-00224.pdfhttp://hdl.handle.net/10204/11520Copyright: 2019, MDPI AGAnalysis of volatile organic compounds in the breath for disease detection and monitoring has gained momentum and clinical significance due to its rapid test results and non-invasiveness, especially for diabetes mellitus (DM). Studies have suggested that breath gases, including acetone, may be related to simultaneous blood glucose (BG) and blood ketone levels in adults with types 2 and 1 diabetes. Detecting altered concentrations of ketones in the breath, blood and urine may be crucial for the diagnosis and monitoring of diabetes mellitus. This study assesses the efficacy of a simple breath test as a non-invasive means of diabetes monitoring in adults with type 2 diabetes mellitus. Human breath samples were collected in Tedlar™ bags and analyzed by headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME/GC-MS). The measurements were compared with capillary BG and blood ketone levels (ß-hydroxybutyrate and acetoacetate) taken at the same time on a single visit to a routine hospital clinic in 30 subjects with type 2 diabetes and 28 control volunteers. Ketone bodies of diabetic subjects showed a significant increase when compared to the control subjects; however, the ketone levels were was controlled in both diabetic and non-diabetic volunteers. Worthy of note, a statistically significant relationship was found between breath acetone and blood acetoacetate (R = 0.89) and between breath acetone and ß-hydroxybutyrate (R = 0.82).enAcetoneAcetoacetateDiabetes mellitusGas chromatography-mass spectrometryHeta-hydroxybutyrateArticleSaasa, V., Beukes, M., Lemmer, Y., & Mwakikunga, B. W. (2019). Blood ketone bodies and breath acetone analysis and their correlations in Type 2 Diabetes Mellitus. http://hdl.handle.net/10204/11520Saasa, Valentine, M Beukes, Yolandy Lemmer, and Bonex W Mwakikunga "Blood ketone bodies and breath acetone analysis and their correlations in Type 2 Diabetes Mellitus." (2019) http://hdl.handle.net/10204/11520Saasa V, Beukes M, Lemmer Y, Mwakikunga BW. Blood ketone bodies and breath acetone analysis and their correlations in Type 2 Diabetes Mellitus. 2019; http://hdl.handle.net/10204/11520.TY - Article AU - Saasa, Valentine AU - Beukes, M AU - Lemmer, Yolandy AU - Mwakikunga, Bonex W AB - Analysis of volatile organic compounds in the breath for disease detection and monitoring has gained momentum and clinical significance due to its rapid test results and non-invasiveness, especially for diabetes mellitus (DM). Studies have suggested that breath gases, including acetone, may be related to simultaneous blood glucose (BG) and blood ketone levels in adults with types 2 and 1 diabetes. Detecting altered concentrations of ketones in the breath, blood and urine may be crucial for the diagnosis and monitoring of diabetes mellitus. This study assesses the efficacy of a simple breath test as a non-invasive means of diabetes monitoring in adults with type 2 diabetes mellitus. Human breath samples were collected in Tedlar™ bags and analyzed by headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME/GC-MS). The measurements were compared with capillary BG and blood ketone levels (ß-hydroxybutyrate and acetoacetate) taken at the same time on a single visit to a routine hospital clinic in 30 subjects with type 2 diabetes and 28 control volunteers. Ketone bodies of diabetic subjects showed a significant increase when compared to the control subjects; however, the ketone levels were was controlled in both diabetic and non-diabetic volunteers. Worthy of note, a statistically significant relationship was found between breath acetone and blood acetoacetate (R = 0.89) and between breath acetone and ß-hydroxybutyrate (R = 0.82). DA - 2019-12 DB - ResearchSpace DP - CSIR KW - Acetone KW - Acetoacetate KW - Diabetes mellitus KW - Gas chromatography-mass spectrometry KW - Heta-hydroxybutyrate LK - https://researchspace.csir.co.za PY - 2019 SM - 2075-4418 T1 - Blood ketone bodies and breath acetone analysis and their correlations in Type 2 Diabetes Mellitus TI - Blood ketone bodies and breath acetone analysis and their correlations in Type 2 Diabetes Mellitus UR - http://hdl.handle.net/10204/11520 ER -