Ncube, ENMathiba, KgamaSteenkamp, Lucia HDubery, IA2022-02-252022-02-252021-10Ncube, E., Mathiba, K., Steenkamp, L.H. & Dubery, I. 2021. Gas chromatographic profiling of the biocatalytic conversion of sclareol to ambradiol by Hyphozyma roseoniger. <i>Biocatalysis and Biotransformation.</i> http://hdl.handle.net/10204/122881024-24221029-2446https://doi.org/10.1080/10242422.2021.1993200http://hdl.handle.net/10204/12288Hyphozyma roseoniger is a filamentous yeast used for its biocatalytic ability to convert sclareol, a plant diterpenoid to ambradiol, an intermediate to ambrafuran, which is a sought-after fixative in the perfume industry. Metabolite profiling is a suitable investigative tool to dissect the biochemical steps involved in the biocatalytic reaction by H. roseoniger from sclareol to ambradiol. H. roseoniger suspensions were grown in batch culture to simulate growth in a bioreactor over a 14-day period. Cells were harvested at stipulated days (phases of growth) using an ethyl acetate extraction procedure. The progress of the bioconversion was monitored using gas chromatography with flame ionisation detection (GC–FID) profiling. Here, decreasing concentrations of sclareol with concomitant increasing concentrations of ambradiol as product and with sclareolide as a putative intermediate, were recorded. While the presence of unidentified peaks were noted in the GC chromatograms, no previously unknown intermediates could be identified in the sequence of sclareol to sclareolide to ambradiol.AbstractenAmbradiolBiocatalysisGas chromatographyHyphozyma roseonigerSclareolArticleNcube, E., Mathiba, K., Steenkamp, L. H., & Dubery, I. (2021). Gas chromatographic profiling of the biocatalytic conversion of sclareol to ambradiol by Hyphozyma roseoniger. <i>Biocatalysis and Biotransformation</i>, http://hdl.handle.net/10204/12288Ncube, EN, Kgama Mathiba, Lucia H Steenkamp, and IA Dubery "Gas chromatographic profiling of the biocatalytic conversion of sclareol to ambradiol by Hyphozyma roseoniger." <i>Biocatalysis and Biotransformation</i> (2021) http://hdl.handle.net/10204/12288Ncube E, Mathiba K, Steenkamp LH, Dubery I. Gas chromatographic profiling of the biocatalytic conversion of sclareol to ambradiol by Hyphozyma roseoniger. Biocatalysis and Biotransformation. 2021; http://hdl.handle.net/10204/12288.TY - Article AU - Ncube, EN AU - Mathiba, Kgama AU - Steenkamp, Lucia H AU - Dubery, IA AB - Hyphozyma roseoniger is a filamentous yeast used for its biocatalytic ability to convert sclareol, a plant diterpenoid to ambradiol, an intermediate to ambrafuran, which is a sought-after fixative in the perfume industry. Metabolite profiling is a suitable investigative tool to dissect the biochemical steps involved in the biocatalytic reaction by H. roseoniger from sclareol to ambradiol. H. roseoniger suspensions were grown in batch culture to simulate growth in a bioreactor over a 14-day period. Cells were harvested at stipulated days (phases of growth) using an ethyl acetate extraction procedure. The progress of the bioconversion was monitored using gas chromatography with flame ionisation detection (GC–FID) profiling. Here, decreasing concentrations of sclareol with concomitant increasing concentrations of ambradiol as product and with sclareolide as a putative intermediate, were recorded. While the presence of unidentified peaks were noted in the GC chromatograms, no previously unknown intermediates could be identified in the sequence of sclareol to sclareolide to ambradiol. DA - 2021-10 DB - ResearchSpace DP - CSIR J1 - Biocatalysis and Biotransformation KW - Ambradiol KW - Biocatalysis KW - Gas chromatography KW - Hyphozyma roseoniger KW - Sclareol LK - https://researchspace.csir.co.za PY - 2021 SM - 1024-2422 SM - 1029-2446 T1 - Gas chromatographic profiling of the biocatalytic conversion of sclareol to ambradiol by Hyphozyma roseoniger TI - Gas chromatographic profiling of the biocatalytic conversion of sclareol to ambradiol by Hyphozyma roseoniger UR - http://hdl.handle.net/10204/12288 ER -25290