Louvel, SMoodley, NSeibert, ISteenkamp, PNthambeleni, RVidal, VMaharaj, VKlimkait, T2013-10-232013-10-232013-05Louvel, S, Moodley, N, Seibert, I, Steenkamp, P, Nthambeleni, R, Vidal, V, Maharaj, V and Klimkait, T. 2013. Identification of compounds from the plant species Alepidea amatymbica active against HIV. South African Journal of Botany, vol. 86, pp 9-140254-6299http://ac.els-cdn.com/S0254629913000240/1-s2.0-S0254629913000240-main.pdf?_tid=25912222-3595-11e3-9dfb-00000aacb35e&acdnat=1381840224_3c78e1e1519414acf5a4daf450044933http://hdl.handle.net/10204/6988Copyright: 2013 Elsevier. This is the Pre/post print. The definitive version is published in South African Journal of Botany, vol. 86, pp 9-14As Alepidea amatymbica is commonly used and accepted asmedicinal plant in South Africa for various indications, the scientific basis of its anecdotally described, putative anti-HIV propertieswas investigated. To this aim, we used an accelerated extraction–purification approach; extracts and therein sub-fractions of A. amatymbica were assessed in a cell-based assay targeting the replication of prototypic CXCR4-tropic (NL4-3) or CCR5-tropic (NL-AD87) HIV-1 strains. Sub-fractions of the extracts were generated through semi-preparative high performance liquid chromatography (HPLC) fractionation into triplicates of 96-well microtitre plates; they were then separately subjected to biological analysis and ultra performance liquid chromatography (UPLC) time-of-flight (TOF) analysis. A correlation plot was generated between the biological and chemical data to identify the biologically active compounds in those fractions that showed significant selective anti-HIV activity. The results indicated that rosmarinic acid was present in the wells that showed promising anti-HIV activity in vitro indicating that this compound is at least in part responsible for the antiviral properties of the A.amatymbica extracts.However, compared to standard retroviral inhibitor the anti-HIV activity of the pure compound was found to be only quite moderate. Nevertheless, the accelerated approach described herein increases the efficiency of screens towards identifying drug candidates much earlier in the discovery stage.enPlant-based inhibitorsHerbal medicineTraditional medicineEthnobotanyVirus profilingAntiretroviral screensArticleLouvel, S., Moodley, N., Seibert, I., Steenkamp, P., Nthambeleni, R., Vidal, V., ... Klimkait, T. (2013). Identification of compounds from the plant species Alepidea amatymbica active against HIV. http://hdl.handle.net/10204/6988Louvel, S, N Moodley, I Seibert, P Steenkamp, R Nthambeleni, V Vidal, V Maharaj, and T Klimkait "Identification of compounds from the plant species Alepidea amatymbica active against HIV." (2013) http://hdl.handle.net/10204/6988Louvel S, Moodley N, Seibert I, Steenkamp P, Nthambeleni R, Vidal V, et al. Identification of compounds from the plant species Alepidea amatymbica active against HIV. 2013; http://hdl.handle.net/10204/6988.TY - Article AU - Louvel, S AU - Moodley, N AU - Seibert, I AU - Steenkamp, P AU - Nthambeleni, R AU - Vidal, V AU - Maharaj, V AU - Klimkait, T AB - As Alepidea amatymbica is commonly used and accepted asmedicinal plant in South Africa for various indications, the scientific basis of its anecdotally described, putative anti-HIV propertieswas investigated. To this aim, we used an accelerated extraction–purification approach; extracts and therein sub-fractions of A. amatymbica were assessed in a cell-based assay targeting the replication of prototypic CXCR4-tropic (NL4-3) or CCR5-tropic (NL-AD87) HIV-1 strains. Sub-fractions of the extracts were generated through semi-preparative high performance liquid chromatography (HPLC) fractionation into triplicates of 96-well microtitre plates; they were then separately subjected to biological analysis and ultra performance liquid chromatography (UPLC) time-of-flight (TOF) analysis. A correlation plot was generated between the biological and chemical data to identify the biologically active compounds in those fractions that showed significant selective anti-HIV activity. The results indicated that rosmarinic acid was present in the wells that showed promising anti-HIV activity in vitro indicating that this compound is at least in part responsible for the antiviral properties of the A.amatymbica extracts.However, compared to standard retroviral inhibitor the anti-HIV activity of the pure compound was found to be only quite moderate. Nevertheless, the accelerated approach described herein increases the efficiency of screens towards identifying drug candidates much earlier in the discovery stage. DA - 2013-05 DB - ResearchSpace DP - CSIR KW - Plant-based inhibitors KW - Herbal medicine KW - Traditional medicine KW - Ethnobotany KW - Virus profiling KW - Antiretroviral screens LK - https://researchspace.csir.co.za PY - 2013 SM - 0254-6299 T1 - Identification of compounds from the plant species Alepidea amatymbica active against HIV TI - Identification of compounds from the plant species Alepidea amatymbica active against HIV UR - http://hdl.handle.net/10204/6988 ER -