Thobakgale, Setumo LManoto, Sello LOmbinda-Lemboumba, SaturninMaaza, MMthunzi-Kufa, Patience2017-11-062017-11-062017-01Thobakgale, S.L. et al. 2017. Efficient femtosecond driven SOX 17 delivery into mouse embryonic stem cells: Differentiation study. Proceedings Volume 10062, Optical Interactions with Tissue and Cells XXVIII; 1006206 (2017), San Francisco, California, United States, 28 January- 2 February 2017978-1-510605-77-0https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10068/100681U/Efficient-femtosecond-driven-SOX-17-delivery-into-mouse-embryonic-stem/10.1117/12.2252255.fullDOI: 10.1117/12.2252255http://hdl.handle.net/10204/9725Copyright: 2017 SPIE. Two items attached. Due to copyright restrictions, the first attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website. The second attachment contains the poster delivered at the conference.Embryonic stem cells have great promise in regenerative medicine because of their ability to self-renew and differentiate into various cell types. Delivery of therapeutic genes into cells has already been achieved using of chemical agents and viral vectors with high transfection efficiencies. However, these methods have also been documented as toxic and in the latter case they can cause latent cell infections. In this study we use femtosecond laser pulses to optically deliver genetic material in mouse embryonic stem cells. Femtosecond laser pulses in contrast to the conventional approach, minimises the risk of unwanted side effects because photons are used to create transient pores on the membrane which allow free entry of molecules with no need for delivery agents. Using an Olympus microscope, fluorescence imaging of the samples post irradiation was performed and decreased expression of stage specific embryonic antigen one (SSEA-1) consistent with on-going cellular differentiation was observed. Our results also show that femtosecond laser pulses were effective in delivering SOX 17 plasmid DNA (pSOX17) which resulted in the differentiation of mouse embryonic stem cells into endoderm cells. We thus concluded that laser transfection of stem cells for the purpose of differentiation, holds potential for applications in tissue engineering as a method of generating new cell lines.enEmbryonic stem cellsESCsMouse embryonic stem cellsmESPlasmid SOX17pSOX17DifferentiationImmunofluorescenceViabilityMorphologyStage-specific embryonic antigen oneSSEA-1phototransfectionConference PresentationThobakgale, S. L., Manoto, S. L., Ombinda-Lemboumba, S., Maaza, M., & Mthunzi-Kufa, P. (2017). Efficient femtosecond driven SOX 17 delivery into mouse embryonic stem cells: Differentiation study. SPIE. http://hdl.handle.net/10204/9725Thobakgale, Setumo L, Sello L Manoto, Saturnin Ombinda-Lemboumba, M Maaza, and Patience Mthunzi-Kufa. "Efficient femtosecond driven SOX 17 delivery into mouse embryonic stem cells: Differentiation study." (2017): http://hdl.handle.net/10204/9725Thobakgale SL, Manoto SL, Ombinda-Lemboumba S, Maaza M, Mthunzi-Kufa P, Efficient femtosecond driven SOX 17 delivery into mouse embryonic stem cells: Differentiation study; SPIE; 2017. http://hdl.handle.net/10204/9725 .TY - Conference Presentation AU - Thobakgale, Setumo L AU - Manoto, Sello L AU - Ombinda-Lemboumba, Saturnin AU - Maaza, M AU - Mthunzi-Kufa, Patience AB - Embryonic stem cells have great promise in regenerative medicine because of their ability to self-renew and differentiate into various cell types. Delivery of therapeutic genes into cells has already been achieved using of chemical agents and viral vectors with high transfection efficiencies. However, these methods have also been documented as toxic and in the latter case they can cause latent cell infections. In this study we use femtosecond laser pulses to optically deliver genetic material in mouse embryonic stem cells. Femtosecond laser pulses in contrast to the conventional approach, minimises the risk of unwanted side effects because photons are used to create transient pores on the membrane which allow free entry of molecules with no need for delivery agents. Using an Olympus microscope, fluorescence imaging of the samples post irradiation was performed and decreased expression of stage specific embryonic antigen one (SSEA-1) consistent with on-going cellular differentiation was observed. Our results also show that femtosecond laser pulses were effective in delivering SOX 17 plasmid DNA (pSOX17) which resulted in the differentiation of mouse embryonic stem cells into endoderm cells. We thus concluded that laser transfection of stem cells for the purpose of differentiation, holds potential for applications in tissue engineering as a method of generating new cell lines. DA - 2017-01 DB - ResearchSpace DP - CSIR KW - Embryonic stem cells KW - ESCs KW - Mouse embryonic stem cells KW - mES KW - Plasmid SOX17 KW - pSOX17 KW - Differentiation KW - Immunofluorescence KW - Viability KW - Morphology KW - Stage-specific embryonic antigen one KW - SSEA-1 KW - phototransfection LK - https://researchspace.csir.co.za PY - 2017 SM - 978-1-510605-77-0 T1 - Efficient femtosecond driven SOX 17 delivery into mouse embryonic stem cells: Differentiation study TI - Efficient femtosecond driven SOX 17 delivery into mouse embryonic stem cells: Differentiation study UR - http://hdl.handle.net/10204/9725 ER -