Josiah, Jideani CGovender, KKGovender, PPCordier, WNell, M2026-04-232026-04-232026-020020-76081097-461Xhttps://doi.org/10.1002/qua.70172Digital Object Identifier (DOI)http://hdl.handle.net/10204/14791Cutaneous squamous cell carcinoma (cSCC) poses a significant therapeutic challenge due to its aggressive nature and recurrence rates. The current treatment 5-fluorouracil (5-FU) is associated with adverse skin reactions. This study investigates cannabidiol (CBD) as a potential alternative therapy for cSCC through an integrated computational and experimental approach. Density functional theory (DFT) using the M06-2X/6-31+G(d,p) basis set revealed that CBD's smaller HOMO–LUMO gap (0.282 eV) compared to 5-FU (0.288 eV) indicates a higher reactivity and potential biological interactions. Cannabidiol exhibits a higher binding affinity toward the CB1 receptor (−9.986 kcal/mol) than 5-FU (−3.760 kcal/mol). Molecular dynamics simulations demonstrate that the CBD–CB1 complex remains stable through hydrogen bonding and hydrophobic interactions. Binding free energy calculations (MM-GBSA) further confirmed CBD's enhanced affinity (−69.696 kcal/mol) over 5-FU (−28.241 kcal/mol). Experimentally, CBD exhibited greater cytotoxicity against A431 cSCC cells with an IC50 of 2.76 μM compared to 5-FU's IC50 of 5.61 μM. These integrated findings suggest that CBD is a promising alternative therapeutic candidate for cSCC, offering superior cytotoxicity and stable molecular interactions compared to 5-FU.FulltextenCutaneous squamous cell carcinomaCannabidiolAlternative cancer therapyMolecular modeling and simulationsCB1 receptor bindingCytotoxicityArticlen/a