Enzymatic synthesis of 5-methyluridine by transglycosylation of guanosine and thymine

Visser, Daniel FGordon, GERBode, MLMathiba, KBrady, D2013-01-302013-01-302012-05Visser, DF, Gordon, GER, Bode, ML, Mathiba, K and Brady, D. 2012. Enzymatic synthesis of 5-methyluridine by transglycosylation of guanosine and thymine. Practical Methods for Biocatalysis and Biotransformations 2. Wiley-Blackwell. Chichester, UK, pp. 235-240978-1-119-99139-7978-1-119-94342-6http://onlinelibrary.wiley.com/book/10.1002/9781119943426http://hdl.handle.net/10204/6497Copyright: 2012 John Wiley & Sons. This is an ABSTRACT ONLY.5-Methyluridine (5-MU) is an intermediate in the synthesis of ß-thymidine and the antiretroviral drugs stavudine (d4T) and zidovudine (AZT)1-3. The enzymatic preparation of 5-MU involves transglycosylation4-6 and avoids the formation of unwanted isomers. The overall transglycosylation reaction effectively converts one nucleoside into another through exchange of the heterocyclic base in the presence of nucleoside phosphorylases.enEnzymatic synthesis5-methyluridineTransglycosylationGuanosineThymineEnzymesEnzymatic synthesis of 5-methyluridine by transglycosylation of guanosine and thymineBook ChapterVisser, D. F., Gordon, G., Bode, M., Mathiba, K., & Brady, D. (2012). Enzymatic synthesis of 5-Methyluridine by transglycosylation of guanosine and thymine., <i>Workflow;5455</i> Wiley-Blackwell. http://hdl.handle.net/10204/6497Visser, Daniel F, GER Gordon, ML Bode, K Mathiba, and D Brady. "Enzymatic synthesis of 5-methyluridine by transglycosylation of guanosine and thymine" In <i>WORKFLOW;5455</i>, n.p.: Wiley-Blackwell. 2012. http://hdl.handle.net/10204/6497.Visser DF, Gordon G, Bode M, Mathiba K, Brady D. Enzymatic synthesis of 5-methyluridine by transglycosylation of guanosine and thymine.. Workflow;5455. [place unknown]: Wiley-Blackwell; 2012. [cited yyyy month dd]. http://hdl.handle.net/10204/6497.TY - Book Chapter AU - Visser, Daniel F AU - Gordon, GER AU - Bode, ML AU - Mathiba, K AU - Brady, D AB - 5-Methyluridine (5-MU) is an intermediate in the synthesis of ß-thymidine and the antiretroviral drugs stavudine (d4T) and zidovudine (AZT)1-3. The enzymatic preparation of 5-MU involves transglycosylation4-6 and avoids the formation of unwanted isomers. The overall transglycosylation reaction effectively converts one nucleoside into another through exchange of the heterocyclic base in the presence of nucleoside phosphorylases. DA - 2012-05 DB - ResearchSpace DP - CSIR KW - Enzymatic synthesis KW - 5-methyluridine KW - Transglycosylation KW - Guanosine KW - Thymine KW - Enzymes LK - https://researchspace.csir.co.za PY - 2012 SM - 978-1-119-99139-7 SM - 978-1-119-94342-6 T1 - Enzymatic synthesis of 5-methyluridine by transglycosylation of guanosine and thymine TI - Enzymatic synthesis of 5-methyluridine by transglycosylation of guanosine and thymine UR - http://hdl.handle.net/10204/6497 ER -