Lalloo, RajeshRamchuran, Santosh ORamduth, DMGorgens, J2007-06-292007-06-292007-02Lalloo, R et al. 2007. Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish. Journal of Applied Microbiology, pp 1-91364-5072http://hdl.handle.net/10204/825https://pubmed.ncbi.nlm.nih.gov/17953558/DOI: 10.1111/j.1365-2672.2007.03360.xCopyright: 2007 Blackwell PublishingNatural isolates obtained from mud sediment and Cyprinus carpio were purified and assessed in vitro for efficacy based on the inhibi-tion of growth of pathogenic Aeromonas hydrophila and the decrease in concentrations of ammonium, nitrite, nitrate and phosphate ions. Based on suitability to predefined characteristics, the isolates B001, B002 and B003 were selected and evaluated in vitro in the presence of Aer. hydrophila and in a pre-liminary in vivo trial with C. carpio. The inhibitory effect on pathogen growth and the decrease in concentrations of waste ions was demonstrated. Based on 16S RNA sequence homology, the isolates were identified as Bacillus subtilis, Bacillus cereus and Bacillus licheniformis, respectively. Isolate B002 did not contain the anthrax virulence plasmids pOX1, pOX2 or the B. cereus enterotoxin. Conclusions: Selected isolates effected synergistic reduction in pathogen load and the concentrations of waste ions in vitro and in vivo and are safe for use in ornamental aquaculture. Significance and Impact of the Study: A new approach for assessment of biological agents was demonstrated and has yielded putative isolates for development into aquaculture products.enAquacultureBacillus sppBacterial pathogensBiological agentWater qualityMud sedimentCyprinus carpioArticleLalloo, R., Ramchuran, S. O., Ramduth, D., & Gorgens, J. (2007). Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish. http://hdl.handle.net/10204/825Lalloo, Rajesh, Santosh O Ramchuran, DM Ramduth, and J Gorgens "Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish." (2007) http://hdl.handle.net/10204/825Lalloo R, Ramchuran SO, Ramduth D, Gorgens J. Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish. 2007; http://hdl.handle.net/10204/825.TY - Article AU - Lalloo, Rajesh AU - Ramchuran, Santosh O AU - Ramduth, DM AU - Gorgens, J AB - Natural isolates obtained from mud sediment and Cyprinus carpio were purified and assessed in vitro for efficacy based on the inhibi-tion of growth of pathogenic Aeromonas hydrophila and the decrease in concentrations of ammonium, nitrite, nitrate and phosphate ions. Based on suitability to predefined characteristics, the isolates B001, B002 and B003 were selected and evaluated in vitro in the presence of Aer. hydrophila and in a pre-liminary in vivo trial with C. carpio. The inhibitory effect on pathogen growth and the decrease in concentrations of waste ions was demonstrated. Based on 16S RNA sequence homology, the isolates were identified as Bacillus subtilis, Bacillus cereus and Bacillus licheniformis, respectively. Isolate B002 did not contain the anthrax virulence plasmids pOX1, pOX2 or the B. cereus enterotoxin. Conclusions: Selected isolates effected synergistic reduction in pathogen load and the concentrations of waste ions in vitro and in vivo and are safe for use in ornamental aquaculture. Significance and Impact of the Study: A new approach for assessment of biological agents was demonstrated and has yielded putative isolates for development into aquaculture products. DA - 2007-02 DB - ResearchSpace DP - CSIR KW - Aquaculture KW - Bacillus spp KW - Bacterial pathogens KW - Biological agent KW - Water quality KW - Mud sediment KW - Cyprinus carpio LK - https://researchspace.csir.co.za PY - 2007 SM - 1364-5072 T1 - Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish TI - Isolation and selection of Bacillus spp as potential biological agents for enhancement of water quality in culture of ornamental fish UR - http://hdl.handle.net/10204/825 ER -