Ncube, ENSteenkamp, Paul AMadala, NEDubery, IA2017-07-282017-07-282016-07Ncube, E.N., Steenkamp, P.A., Madala, N.E. et al. 2016. Chlorogenic acids biosynthesis in Centella asiatica cells is not stimulated by salicylic acid manipulation. Applied Biochemistry and Biotechnology, vol. 179(5): 685-696. DOI: 10.1007/s12010-016-2024-90273-2289DOI: 10.1007/s12010-016-2024-9https://link.springer.com/article/10.1007/s12010-016-2024-9http://hdl.handle.net/10204/9391Copyright: 2016 Springer. Due to copyright restrictions, the attached PDF file contains the accepted version of the published article. For access to the full text item, kindly consult the publisher's website.Exogenous application of synthetic and natural elicitors of plant defence has been shown to result in mass production of secondary metabolites with nutraceuticals properties in cultured cells. In particular, salicylic acid (SA) treatment has been reported to induce the production of phenylpropanoids, including cinnamic acid derivatives bound to quinic acid (chlorogenic acids). Centella asiatica is an important medicinal plant with several therapeutic properties owing to its wide spectrum of secondary metabolites. We investigated the effect of SA on C. asiatica cells by monitoring perturbation of chlorogenic acids in particular. Different concentrations of SA were used to treat C. asiatica cells, and extracts from both treated and untreated cells were analysed using an optimised UHPLC-QTOF-MS/MS method. Semi-targeted multivariate data analyses with the aid of principal component analysis (PCA) and orthogonal projection to latent structures-discriminant analysis (OPLS-DA) revealed a concentration-dependent metabolic response. Surprisingly, a range of chlorogenic acid derivatives were found to be downregulated as a consequence of SA treatment. Moreover, irbic acid (3,5-O-dicaffeoyl-4-O-malonilquinic acid) was found to be a dominant CGA in C. asiatica cells, although the SA treatment also had a negative effect on its concentration. Overall SA treatment was found to be an ineffective elicitor of CGA production in cultured C. asiatica cells.enCaffeoyl quinic acidsCentella asiaticaChlorogenic acidsElicitationSalicylic acidNeuroprotectionArticleNcube, E., Steenkamp, P. A., Madala, N., & Dubery, I. (2016). Chlorogenic acids biosynthesis in Centella asiatica cells is not stimulated by salicylic acid manipulation. http://hdl.handle.net/10204/9391Ncube, EN, Paul A Steenkamp, NE Madala, and IA Dubery "Chlorogenic acids biosynthesis in Centella asiatica cells is not stimulated by salicylic acid manipulation." (2016) http://hdl.handle.net/10204/9391Ncube E, Steenkamp PA, Madala N, Dubery I. Chlorogenic acids biosynthesis in Centella asiatica cells is not stimulated by salicylic acid manipulation. 2016; http://hdl.handle.net/10204/9391.TY - Article AU - Ncube, EN AU - Steenkamp, Paul A AU - Madala, NE AU - Dubery, IA AB - Exogenous application of synthetic and natural elicitors of plant defence has been shown to result in mass production of secondary metabolites with nutraceuticals properties in cultured cells. In particular, salicylic acid (SA) treatment has been reported to induce the production of phenylpropanoids, including cinnamic acid derivatives bound to quinic acid (chlorogenic acids). Centella asiatica is an important medicinal plant with several therapeutic properties owing to its wide spectrum of secondary metabolites. We investigated the effect of SA on C. asiatica cells by monitoring perturbation of chlorogenic acids in particular. Different concentrations of SA were used to treat C. asiatica cells, and extracts from both treated and untreated cells were analysed using an optimised UHPLC-QTOF-MS/MS method. Semi-targeted multivariate data analyses with the aid of principal component analysis (PCA) and orthogonal projection to latent structures-discriminant analysis (OPLS-DA) revealed a concentration-dependent metabolic response. Surprisingly, a range of chlorogenic acid derivatives were found to be downregulated as a consequence of SA treatment. Moreover, irbic acid (3,5-O-dicaffeoyl-4-O-malonilquinic acid) was found to be a dominant CGA in C. asiatica cells, although the SA treatment also had a negative effect on its concentration. Overall SA treatment was found to be an ineffective elicitor of CGA production in cultured C. asiatica cells. DA - 2016-07 DB - ResearchSpace DP - CSIR KW - Caffeoyl quinic acids KW - Centella asiatica KW - Chlorogenic acids KW - Elicitation KW - Salicylic acid KW - Neuroprotection LK - https://researchspace.csir.co.za PY - 2016 SM - 0273-2289 T1 - Chlorogenic acids biosynthesis in Centella asiatica cells is not stimulated by salicylic acid manipulation TI - Chlorogenic acids biosynthesis in Centella asiatica cells is not stimulated by salicylic acid manipulation UR - http://hdl.handle.net/10204/9391 ER -