Isoniazid-loaded orodispersible strips: Methodical design, optimization and in vitro-in silico characterization

Adeleke, Oluwatoyin ATsai, PKarry, KMMonama, Nkwe OMichniak-Kohn, BB2021-11-262021-11-262021-08Adeleke, O.A., Tsai, P., Karry, K., Monama, N.O. & Michniak-Kohn, B. 2021. Isoniazid-loaded orodispersible strips: Methodical design, optimization and in vitro-in silico characterization. International Journal of Pharmaceutics, 25. http://hdl.handle.net/10204/121760378-51731873-3476DOI: 10.1016/j.ijpharm.2018.06.004http://hdl.handle.net/10204/12176Drug treatment remains the most effective global approach to managing and preventing tuberculosis. This work focuses on formulating and evaluating an optimized polyvinyl alcohol-polyethylene glycol based orodispersible strip containing isoniazid, a first-line anti-tubercular agent. A solvent casting method guided through a Taguchi experimental design was employed in the fabrication, optimization and characterization of the orodispersible strip. The optimized strip was physically amalgamated with a monolayer, uniformly distributed surface geometry. It was 159.2 ± 3.0 µm thick, weighed 36.9 ± 0.3 mg, had an isoniazid load of 99.5 ± 0.8%w/w, disintegration and dissolution times of 17.6 ± 0.9 s and 5.5 ± 0.1 min respectively. In vitro crystallinity, thermal measurements and in silico thermodynamic predictions confirmed the strip's intrinsic miscibility, thermodynamic stability and amorphous nature. A Korsmeyer-Peppas (r = 0.99; n > 1 = 1.07) fitted kinetics typified by an initial burst release of 49.4 ± 1.9% at 4 min and a total of 99.8 ± 3.3% at 30 min was noted. Ex vivo isoniazid permeation through porcine buccal mucosa was bi-phasic and characterized by a 50.4 ± 3.8% surge and 95.6 ± 2.9% at 5 and 120 min respectively. The strip was physicomechanically robust, environmentally stable and non-cytotoxic.VideoenBuccal absorptionExperimental designIsoniazidMonolayer filmOrodispersible stripTuberculosisIsoniazid-loaded orodispersible strips: Methodical design, optimization and in vitro-in silico characterizationArticleAdeleke, O. A., Tsai, P., Karry, K., Monama, N. O., & Michniak-Kohn, B. (2021). Isoniazid-loaded orodispersible strips: Methodical design, optimization and in vitro-in silico characterization. International Journal of Pharmaceutics, 25, http://hdl.handle.net/10204/12176Adeleke, Oluwatoyin A, P Tsai, KM Karry, Nkwe O Monama, and BB Michniak-Kohn "Isoniazid-loaded orodispersible strips: Methodical design, optimization and in vitro-in silico characterization." International Journal of Pharmaceutics, 25 (2021) http://hdl.handle.net/10204/12176Adeleke OA, Tsai P, Karry K, Monama NO, Michniak-Kohn B. Isoniazid-loaded orodispersible strips: Methodical design, optimization and in vitro-in silico characterization. International Journal of Pharmaceutics, 25. 2021; http://hdl.handle.net/10204/12176.TY - Article AU - Adeleke, Oluwatoyin A AU - Tsai, P AU - Karry, KM AU - Monama, Nkwe O AU - Michniak-Kohn, BB AB - Drug treatment remains the most effective global approach to managing and preventing tuberculosis. This work focuses on formulating and evaluating an optimized polyvinyl alcohol-polyethylene glycol based orodispersible strip containing isoniazid, a first-line anti-tubercular agent. A solvent casting method guided through a Taguchi experimental design was employed in the fabrication, optimization and characterization of the orodispersible strip. The optimized strip was physically amalgamated with a monolayer, uniformly distributed surface geometry. It was 159.2 ± 3.0 µm thick, weighed 36.9 ± 0.3 mg, had an isoniazid load of 99.5 ± 0.8%w/w, disintegration and dissolution times of 17.6 ± 0.9 s and 5.5 ± 0.1 min respectively. In vitro crystallinity, thermal measurements and in silico thermodynamic predictions confirmed the strip's intrinsic miscibility, thermodynamic stability and amorphous nature. A Korsmeyer-Peppas (r = 0.99; n > 1 = 1.07) fitted kinetics typified by an initial burst release of 49.4 ± 1.9% at 4 min and a total of 99.8 ± 3.3% at 30 min was noted. Ex vivo isoniazid permeation through porcine buccal mucosa was bi-phasic and characterized by a 50.4 ± 3.8% surge and 95.6 ± 2.9% at 5 and 120 min respectively. The strip was physicomechanically robust, environmentally stable and non-cytotoxic. DA - 2021-08 DB - ResearchSpace DP - CSIR J1 - International Journal of Pharmaceutics, 25 KW - Buccal absorption KW - Experimental design KW - Isoniazid KW - Monolayer film KW - Orodispersible strip KW - Tuberculosis LK - https://researchspace.csir.co.za PY - 2021 SM - 0378-5173 SM - 1873-3476 T1 - Isoniazid-loaded orodispersible strips: Methodical design, optimization and in vitro-in silico characterization TI - Isoniazid-loaded orodispersible strips: Methodical design, optimization and in vitro-in silico characterization UR - http://hdl.handle.net/10204/12176 ER -25111