Wesley-Smith, JBerjak, PPammenter, NWWalters, Chavon R2014-09-052014-09-052014-03Wesley-Smith, J, Berjak, P, Pammenter, N.W and Walters, C.R. 2014. Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures. Annals of Botany, vol. 113(4), pp 695-7090305-7364http://aob.oxfordjournals.org/content/early/2013/12/23/aob.mct284.abstracthttp://hdl.handle.net/10204/7649Copyright: 2014 Oxford University Press. This is an ABSTRACT ONLY. The definitive version is published in Annals Botany, vol. 113(4), pp 695-709Cryopreservation is the only long-term conservation strategy available for germplasm of recalcitrant-seeded species. Efforts to cryopreserve this form of germplasm are hampered by potentially lethal intracellular freezing events; thus, it is important to understand the relationships among cryo-exposure techniques, water content, structure and survival.enAcer saccharinumCooling rateCryopreservationElectron microscopyEmbryonic axesFreeze-fractureFreez-substitutionIce crystal distributionIce crystal sizeRecalcitrant seedsWarming rateWater contentArticleWesley-Smith, J., Berjak, P., Pammenter, N., & Walters, C. R. (2014). Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures. http://hdl.handle.net/10204/7649Wesley-Smith, J, P Berjak, NW Pammenter, and Chavon R Walters "Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures." (2014) http://hdl.handle.net/10204/7649Wesley-Smith J, Berjak P, Pammenter N, Walters CR. Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures. 2014; http://hdl.handle.net/10204/7649.TY - Article AU - Wesley-Smith, J AU - Berjak, P AU - Pammenter, NW AU - Walters, Chavon R AB - Cryopreservation is the only long-term conservation strategy available for germplasm of recalcitrant-seeded species. Efforts to cryopreserve this form of germplasm are hampered by potentially lethal intracellular freezing events; thus, it is important to understand the relationships among cryo-exposure techniques, water content, structure and survival. DA - 2014-03 DB - ResearchSpace DP - CSIR KW - Acer saccharinum KW - Cooling rate KW - Cryopreservation KW - Electron microscopy KW - Embryonic axes KW - Freeze-fracture KW - Freez-substitution KW - Ice crystal distribution KW - Ice crystal size KW - Recalcitrant seeds KW - Warming rate KW - Water content LK - https://researchspace.csir.co.za PY - 2014 SM - 0305-7364 T1 - Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures TI - Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures UR - http://hdl.handle.net/10204/7649 ER -