Maphanga, Charles PManoto, Sello LOmbinda-Lemboumba, SaturninMthunzi-Kufa, Patience2021-04-132021-04-132021-03Maphanga, C.P., Manoto, S.L., Ombinda-Lemboumba, S. & Mthunzi-Kufa, P. 2021. Detection of mycobacterium tuberculosis using gold nanoparticles conjugated to TB antibodies. http://hdl.handle.net/10204/11985 .0277-786Xhttps://doi.org/10.1117/12.2578789https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11651/116510X/Detection-of-mycobacterium-tuberculosis-using-gold-nanoparticles-conjugated-to-TB/10.1117/12.2578789.full?SSO=1http://hdl.handle.net/10204/11985In recent years, conjugated nanoparticles have gained significant applications in diagnostics, particularly gold nanoparticles (AuNPs). When functionalized with antibodies, AuNPs can selectively interact with cells and biomolecules. The conjugation of biomolecules to AuNPs has been achieved using a variety of techniques, one such approach is the covalent coupling method used in the current study. Generally, in diagnostics, the conjugation of different moieties such as antibodies to the AuNPs widens their applications and provides them with new or enhanced properties. Due to their high specificity and diversity, antibodies are widely used to provide specificity and bioactivity to AuNPs, particularly for biosensor applications. Localized surface plasmon resonance (LSPR) has emerged as a leader among label-free biosensing techniques because it offers sensitive, robust, and rapid detection of biological analytes. Biomolecular adsorptions on AuNPs surface increases the dielectric constant and change the intensities and the wavelengths of the LSPR band associated with AuNPs. As a result, the adsorptions of biomolecules onto surfaces of this AuNPs can be monitored by measuring the absorption spectra of the AuNPs. In this study, TB antibodies were covalently conjugated to AuNPs and used to detect mycolic acid TB antigens at various concentrations. Characterization of the AuNPs was done using transmission electron microscopy (TEM) while the biomolecular interaction between TB antibodies and the antigen was measured using LSPR. From our findings, it was realised that the use of antibodyconjugated AuNPs enhanced the detection of the analyte even at low concentrations of the analyte.AbstractenAuNPsBiosensingGold nanoparticlesLocalized surface plasmon resonanceLSPRMycolic acidConference PresentationMaphanga, C. P., Manoto, S. L., Ombinda-Lemboumba, S., & Mthunzi-Kufa, P. (2021). Detection of mycobacterium tuberculosis using gold nanoparticles conjugated to TB antibodies. http://hdl.handle.net/10204/11985Maphanga, Charles P, Sello L Manoto, Saturnin Ombinda-Lemboumba, and Patience Mthunzi-Kufa. "Detection of mycobacterium tuberculosis using gold nanoparticles conjugated to TB antibodies." <i>Proceedings Volume 11651, Optical Diagnostics and Sensing XXI: Toward Point-of-Care Diagnostics, 6 - 11 March 2021</i> (2021): http://hdl.handle.net/10204/11985Maphanga CP, Manoto SL, Ombinda-Lemboumba S, Mthunzi-Kufa P, Detection of mycobacterium tuberculosis using gold nanoparticles conjugated to TB antibodies; 2021. http://hdl.handle.net/10204/11985 .TY - Conference Presentation AU - Maphanga, Charles P AU - Manoto, Sello L AU - Ombinda-Lemboumba, Saturnin AU - Mthunzi-Kufa, Patience AB - In recent years, conjugated nanoparticles have gained significant applications in diagnostics, particularly gold nanoparticles (AuNPs). When functionalized with antibodies, AuNPs can selectively interact with cells and biomolecules. The conjugation of biomolecules to AuNPs has been achieved using a variety of techniques, one such approach is the covalent coupling method used in the current study. Generally, in diagnostics, the conjugation of different moieties such as antibodies to the AuNPs widens their applications and provides them with new or enhanced properties. Due to their high specificity and diversity, antibodies are widely used to provide specificity and bioactivity to AuNPs, particularly for biosensor applications. Localized surface plasmon resonance (LSPR) has emerged as a leader among label-free biosensing techniques because it offers sensitive, robust, and rapid detection of biological analytes. Biomolecular adsorptions on AuNPs surface increases the dielectric constant and change the intensities and the wavelengths of the LSPR band associated with AuNPs. As a result, the adsorptions of biomolecules onto surfaces of this AuNPs can be monitored by measuring the absorption spectra of the AuNPs. In this study, TB antibodies were covalently conjugated to AuNPs and used to detect mycolic acid TB antigens at various concentrations. Characterization of the AuNPs was done using transmission electron microscopy (TEM) while the biomolecular interaction between TB antibodies and the antigen was measured using LSPR. From our findings, it was realised that the use of antibodyconjugated AuNPs enhanced the detection of the analyte even at low concentrations of the analyte. DA - 2021-03 DB - ResearchSpace DP - CSIR J1 - Proceedings Volume 11651, Optical Diagnostics and Sensing XXI: Toward Point-of-Care Diagnostics, 6 - 11 March 2021 KW - AuNPs KW - Biosensing KW - Gold nanoparticles KW - Localized surface plasmon resonance KW - LSPR KW - Mycolic acid LK - https://researchspace.csir.co.za PY - 2021 SM - 0277-786X T1 - Detection of mycobacterium tuberculosis using gold nanoparticles conjugated to TB antibodies TI - Detection of mycobacterium tuberculosis using gold nanoparticles conjugated to TB antibodies UR - http://hdl.handle.net/10204/11985 ER -24376