Mcotshana, Zenande KSThwala, Nomcebo LOmbinda-Lemboumba, SaturninRamokolo, Lesiba RLugongolo, Masixole YVan Steen, EMthunzi-Kufa, Patience2024-05-282024-05-282024-01Mcotshana, Z.K., Thwala, N.L., Ombinda-Lemboumba, S., Ramokolo, L.R., Lugongolo, M.Y., Van Steen, E. & Mthunzi-Kufa, P. 2024. Detection and quantification of iodine in biological fluids using photonic-based systems: UV-Vis and Transmission spectroscopy. http://hdl.handle.net/10204/13682 .9781510669031DOI:10.1117/12.30024121605-7422http://hdl.handle.net/10204/13682Iodine is a crucial trace element that occurs in minute amounts in nature and is necessary for the development of bones, thyroid function, and several metabolic processes. Iodine deficiency, also known as hypothyroidism, affects millions of individuals worldwide, and an overabundance of iodine in the body is known as hyperthyroidism. The early identification of iodine with high sensitivity and selectivity is crucial for physiological impact since the abnormalities caused by iodine disorder can increase the frequency of mortality and mental impairments. This work aims to detect iodine using UV-Vis and Transmission spectroscopy and utilizing selenium nanoparticles as a probe. Selenium nanoparticles (SeNPs) were synthesized by ND: YAG laser method and characterized by Dynamic light scattering (DLS), and High-resolution transmission electron microscopy (HRTEM), while the conjugation of iodine to SeNPs was confirmed by Ultraviolet-visible (UV-vis) spectroscopy. For iodine detection, UV-Vis and Transmission spectroscopy were used and compared and the synthesized colloidal and spherical selenium nanoparticles were utilized as a probe to detect iodine. The absorption peaks and a red shift for SeNPs changed upon the reaction with iodine and this shift may allow for the estimation of iodine concentration. The two methods will enable the detection and monitoring of iodine at different concentrations in the body thus preventing the onset of iodine-related diseases.AbstractenIodineSelenium nanoparticlesUV-visTransmission spectroscopyConference PresentationMcotshana, Z. K., Thwala, N. L., Ombinda-Lemboumba, S., Ramokolo, L. R., Lugongolo, M. Y., Van Steen, E., & Mthunzi-Kufa, P. (2024). Detection and quantification of iodine in biological fluids using photonic-based systems: UV-Vis and Transmission spectroscopy. http://hdl.handle.net/10204/13682Mcotshana, Zenande KS, Nomcebo L Thwala, Saturnin Ombinda-Lemboumba, Lesiba R Ramokolo, Masixole Y Lugongolo, E Van Steen, and Patience Mthunzi-Kufa. "Detection and quantification of iodine in biological fluids using photonic-based systems: UV-Vis and Transmission spectroscopy." <i>Proceedings of SPIE, Volume 12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases, California, United States, 29-30 January 2024</i> (2024): http://hdl.handle.net/10204/13682Mcotshana ZK, Thwala NL, Ombinda-Lemboumba S, Ramokolo LR, Lugongolo MY, Van Steen E, et al, Detection and quantification of iodine in biological fluids using photonic-based systems: UV-Vis and Transmission spectroscopy; 2024. http://hdl.handle.net/10204/13682 .TY - Conference Presentation AU - Mcotshana, Zenande KS AU - Thwala, Nomcebo L AU - Ombinda-Lemboumba, Saturnin AU - Ramokolo, Lesiba R AU - Lugongolo, Masixole Y AU - Van Steen, E AU - Mthunzi-Kufa, Patience AB - Iodine is a crucial trace element that occurs in minute amounts in nature and is necessary for the development of bones, thyroid function, and several metabolic processes. Iodine deficiency, also known as hypothyroidism, affects millions of individuals worldwide, and an overabundance of iodine in the body is known as hyperthyroidism. The early identification of iodine with high sensitivity and selectivity is crucial for physiological impact since the abnormalities caused by iodine disorder can increase the frequency of mortality and mental impairments. This work aims to detect iodine using UV-Vis and Transmission spectroscopy and utilizing selenium nanoparticles as a probe. Selenium nanoparticles (SeNPs) were synthesized by ND: YAG laser method and characterized by Dynamic light scattering (DLS), and High-resolution transmission electron microscopy (HRTEM), while the conjugation of iodine to SeNPs was confirmed by Ultraviolet-visible (UV-vis) spectroscopy. For iodine detection, UV-Vis and Transmission spectroscopy were used and compared and the synthesized colloidal and spherical selenium nanoparticles were utilized as a probe to detect iodine. The absorption peaks and a red shift for SeNPs changed upon the reaction with iodine and this shift may allow for the estimation of iodine concentration. The two methods will enable the detection and monitoring of iodine at different concentrations in the body thus preventing the onset of iodine-related diseases. DA - 2024-01 DB - ResearchSpace DP - CSIR J1 - Proceedings of SPIE, Volume 12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases, California, United States, 29-30 January 2024 KW - Iodine KW - Selenium nanoparticles KW - UV-vis KW - Transmission spectroscopy LK - https://researchspace.csir.co.za PY - 2024 SM - 9781510669031 SM - DOI:10.1117/12.3002412 SM - 1605-7422 T1 - Detection and quantification of iodine in biological fluids using photonic-based systems: UV-Vis and Transmission spectroscopy TI - Detection and quantification of iodine in biological fluids using photonic-based systems: UV-Vis and Transmission spectroscopy UR - http://hdl.handle.net/10204/13682 ER -27815