Byaruhanga, YBEmmambux, MNBelton, PSWellner, NNg, KGTaylor, JRN2007-06-122007-06-122006-06-14Byaruhanga, YB, et al. 2006. Alteration of kafirin and kafirin film structure by heating with microwave energy and tannin complexation. Journal of Agricultural and Food Chemistry, vol. 54(12), pp 4198-42070021-8561http://hdl.handle.net/10204/593Copyright: 2006 American Chemical SocietyHeating with microwave energy and tannin complexation of kafirin both increase the tensile strength of cast kafirin bioplastic films. The effects of these treatments on the molecular structure of kafirin and of kafirin in the film were investigated. SDS-PAGE of heated wet kafirin showed an increase in kafirin oligomers. Disulfide groups increased in heated kafirin and in films made from the heated kafirin. Fourier transform infrared (FTIR) spectroscopy of heated kafirin and films made from the heated kafirin indicated an increase in beta-sheet conformation. In contrast, kafirin complexation with tannic acid (TA) and sorghum condensed tannin (SCT) resulted in a slight decrease in beta-sheet conformation in the kafirin and a larger decrease in the kafirin in the films. Raman spectroscopy showed that, with TA, there was a shift in peak from 1710 to 1728 cm(-1) for kafirin-tannic acid complexes, indicating kafirin and tannic acid interaction. The protein conformational changes presumably facilitated cross-linking between kafirin molecules and/or between kafirin and the tannins. Thus, although both heating with microwave energy and tannin complexation cause cross-linking of kafirin to increase film tensile strength, their effects on kafirin structure appear to be differentenSDS-PAGEFTIRFourier transform infraredBioplastic filmsProtein secondary structureDisulfide bondsKafirinChemical sciencesArticleByaruhanga, Y., Emmambux, M., Belton, P., Wellner, N., Ng, K., & Taylor, J. (2006). Alteration of kafirin and kafirin film structure by heating with microwave energy and tannin complexation. http://hdl.handle.net/10204/593Byaruhanga, YB, MN Emmambux, PS Belton, N Wellner, KG Ng, and JRN Taylor "Alteration of kafirin and kafirin film structure by heating with microwave energy and tannin complexation." (2006) http://hdl.handle.net/10204/593Byaruhanga Y, Emmambux M, Belton P, Wellner N, Ng K, Taylor J. Alteration of kafirin and kafirin film structure by heating with microwave energy and tannin complexation. 2006; http://hdl.handle.net/10204/593.TY - Article AU - Byaruhanga, YB AU - Emmambux, MN AU - Belton, PS AU - Wellner, N AU - Ng, KG AU - Taylor, JRN AB - Heating with microwave energy and tannin complexation of kafirin both increase the tensile strength of cast kafirin bioplastic films. The effects of these treatments on the molecular structure of kafirin and of kafirin in the film were investigated. SDS-PAGE of heated wet kafirin showed an increase in kafirin oligomers. Disulfide groups increased in heated kafirin and in films made from the heated kafirin. Fourier transform infrared (FTIR) spectroscopy of heated kafirin and films made from the heated kafirin indicated an increase in beta-sheet conformation. In contrast, kafirin complexation with tannic acid (TA) and sorghum condensed tannin (SCT) resulted in a slight decrease in beta-sheet conformation in the kafirin and a larger decrease in the kafirin in the films. Raman spectroscopy showed that, with TA, there was a shift in peak from 1710 to 1728 cm(-1) for kafirin-tannic acid complexes, indicating kafirin and tannic acid interaction. The protein conformational changes presumably facilitated cross-linking between kafirin molecules and/or between kafirin and the tannins. Thus, although both heating with microwave energy and tannin complexation cause cross-linking of kafirin to increase film tensile strength, their effects on kafirin structure appear to be different DA - 2006-06-14 DB - ResearchSpace DP - CSIR KW - SDS-PAGE KW - FTIR KW - Fourier transform infrared KW - Bioplastic films KW - Protein secondary structure KW - Disulfide bonds KW - Kafirin KW - Chemical sciences LK - https://researchspace.csir.co.za PY - 2006 SM - 0021-8561 T1 - Alteration of kafirin and kafirin film structure by heating with microwave energy and tannin complexation TI - Alteration of kafirin and kafirin film structure by heating with microwave energy and tannin complexation UR - http://hdl.handle.net/10204/593 ER -