Crampton, Michael C2010-09-282010-09-282010-07Crampton, M.C. 2010. Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. 11th International Symposium of the Genetics of Industrial Microorganisms, Melbourne, Australia, June 28- July 1, 2010, pp 18http://hdl.handle.net/10204/438111th International Symposium of the Genetics of Industrial Microorganisms, Melbourne, Australia, June 28- July 1 2010This presentation focused on the transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. It concludes to a successful implementation of a high throughput mRNA sandwich hybridisation quantification assay. It also found that B. halodurans sD promoter regulated in a similar fashion in complex media as B. subtilis, that vector choice influences transcript levels, that flgM-null mutant improves transcript levels but not protein levels and that peptide production follows transcript profile for LB.enHeterologous genesBacillus haloduransGeneticsMicroorganismsIndustrial microorganismsGenetics of Industrial Microorganisms (GIM 2010)Conference PresentationCrampton, M. C. (2010). Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. CSIR. http://hdl.handle.net/10204/4381Crampton, Michael C. "Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans." (2010): http://hdl.handle.net/10204/4381Crampton MC, Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans; CSIR; 2010. http://hdl.handle.net/10204/4381 .TY - Conference Presentation AU - Crampton, Michael C AB - This presentation focused on the transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. It concludes to a successful implementation of a high throughput mRNA sandwich hybridisation quantification assay. It also found that B. halodurans sD promoter regulated in a similar fashion in complex media as B. subtilis, that vector choice influences transcript levels, that flgM-null mutant improves transcript levels but not protein levels and that peptide production follows transcript profile for LB. DA - 2010-07 DB - ResearchSpace DP - CSIR KW - Heterologous genes KW - Bacillus halodurans KW - Genetics KW - Microorganisms KW - Industrial microorganisms KW - Genetics of Industrial Microorganisms (GIM 2010) LK - https://researchspace.csir.co.za PY - 2010 T1 - Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans TI - Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans UR - http://hdl.handle.net/10204/4381 ER -