Kahari, DKoorsen, GStoychev, Stoyan HChipeta, ZLabuschagne, MVan Zyl, PNthangeni, B2009-01-212009-01-212008-11Kahari, D, Koorsen, G, Stoychev, S et al. 2008. Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica. Science Real and Relevant: 2nd CSIR Biennial Conference, CSIR International Convention Centre Pretoria, 17 & 18 November 2008, pp 1http://hdl.handle.net/10204/2866Science Real and Relevant: 2nd CSIR Biennial Conference, CSIR International Convention Centre Pretoria, 17 & 18 November 2008The CSIR Biosciences researchers are developing a platform for robust techniques for glyco-profiling of therapeutic glycoproteins. The extracellular lipase (Lip2) (Aloulou, A et al;2007) was used as a model in establishing and evaluating glycan profiling techniques. The gene encoding Lip2 was cloned as a C-terminally His-tagged protein, expressed in Yarrowia lipolytica (Madzak, C et al;2004) and the glycan composition of the purified protein was analysed by HPLC and MALDITOF. The HPLC techniques was also used to profile the glycan composition of EPO expressed in Yarrowia lipolytica yeast.enProteinYarrowia lipolytica yeastN-linked glycansConference PresentationKahari, D., Koorsen, G., Stoychev, S. H., Chipeta, Z., Labuschagne, M., Van Zyl, P., & Nthangeni, B. (2008). Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica. CSIR. http://hdl.handle.net/10204/2866Kahari, D, G Koorsen, Stoyan H Stoychev, Z Chipeta, M Labuschagne, P Van Zyl, and B Nthangeni. "Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica." (2008): http://hdl.handle.net/10204/2866Kahari D, Koorsen G, Stoychev SH, Chipeta Z, Labuschagne M, Van Zyl P, et al, Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica; CSIR; 2008. http://hdl.handle.net/10204/2866 .TY - Conference Presentation AU - Kahari, D AU - Koorsen, G AU - Stoychev, Stoyan H AU - Chipeta, Z AU - Labuschagne, M AU - Van Zyl, P AU - Nthangeni, B AB - The CSIR Biosciences researchers are developing a platform for robust techniques for glyco-profiling of therapeutic glycoproteins. The extracellular lipase (Lip2) (Aloulou, A et al;2007) was used as a model in establishing and evaluating glycan profiling techniques. The gene encoding Lip2 was cloned as a C-terminally His-tagged protein, expressed in Yarrowia lipolytica (Madzak, C et al;2004) and the glycan composition of the purified protein was analysed by HPLC and MALDITOF. The HPLC techniques was also used to profile the glycan composition of EPO expressed in Yarrowia lipolytica yeast. DA - 2008-11 DB - ResearchSpace DP - CSIR KW - Protein KW - Yarrowia lipolytica yeast KW - N-linked glycans LK - https://researchspace.csir.co.za PY - 2008 T1 - Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica TI - Glycoprofiling of N-linked glycans of erythropoietin therapeutic protein expressed in Yarrowia lipolytica UR - http://hdl.handle.net/10204/2866 ER -