Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197

Roth, Robyn LVan Zyl, Petrus JTsekoa, Tsepo LStoychev, Stoyan HMamputha, SiphoButhelezi, Sindisiwe GCrampton, Michael C2017-08-302017-08-302017-04Roth, R.L., Van Zyl, P.J., Tsekoa, T.L. et al. 2017. Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197. Journal of Applied Microbiology, vol. 122: 1402-1411. DOI: 10.1111/jam.134411364-5072DOI: 10.1111/jam.13441http://onlinelibrary.wiley.com/doi/10.1111/jam.13441/fullhttp://hdl.handle.net/10204/9510Copyright: 2017 Elsevier. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website.The aim of this article is to investigate the production of soluble cross-reacting material 197 (CRM(sub197)) in Escherichia coli, a safe and effective T-cell-dependent protein carrier for polysaccharides used in the manufacture and application of multivalent conjugate vaccines.enEscherichia coliCRM197 productionCo-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197ArticleRoth, R. L., Van Zyl, P. J., Tsekoa, T. L., Stoychev, S. H., Mamputha, S., Buthelezi, S. G., & Crampton, M. C. (2017). Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197. http://hdl.handle.net/10204/9510Roth, Robyn L, Petrus J Van Zyl, Tsepo L Tsekoa, Stoyan H Stoychev, Sipho Mamputha, Sindisiwe G Buthelezi, and Michael C Crampton "Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197." (2017) http://hdl.handle.net/10204/9510Roth RL, Van Zyl PJ, Tsekoa TL, Stoychev SH, Mamputha S, Buthelezi SG, et al. Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197. 2017; http://hdl.handle.net/10204/9510.TY - Article AU - Roth, Robyn L AU - Van Zyl, Petrus J AU - Tsekoa, Tsepo L AU - Stoychev, Stoyan H AU - Mamputha, Sipho AU - Buthelezi, Sindisiwe G AU - Crampton, Michael C AB - The aim of this article is to investigate the production of soluble cross-reacting material 197 (CRM(sub197)) in Escherichia coli, a safe and effective T-cell-dependent protein carrier for polysaccharides used in the manufacture and application of multivalent conjugate vaccines. DA - 2017-04 DB - ResearchSpace DP - CSIR KW - Escherichia coli KW - CRM197 production LK - https://researchspace.csir.co.za PY - 2017 SM - 1364-5072 T1 - Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197 TI - Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197 UR - http://hdl.handle.net/10204/9510 ER -