Thantsha, MSLabuschagne, Philip WMamvura, CI2014-04-102014-04-102014-02Thantsha, M.S, Labuschagne, P.W and Mamvura, C.I. 2014. Supercritical CO2 interpolymer complex encapsulation improves heat stability of probiotic bifidobacteria. World Journal of Microbiology and Biotechnology, vol. 30(2), pp 479-4860959-3993http://download.springer.com/static/pdf/546/art%253A10.1007%252Fs11274-013-1465-3.pdf?auth66=1396707115_d9e60a3c6228782d002694d0f02b37b4&ext=.pdfhttp://hdl.handle.net/10204/7351Copyright: 2014 Springer. Thi is an ABSTRACT ONLY. The definitive version is published in World Journal of Microbiology and Biotechnology, vol. 30(2), pp 479-486The probiotic industry faces the challenge of retention of probiotic culture viability as numbers of these cells within their products inevitably decrease over time. In order to retain probiotic viability levels above the therapeutic minimum over the duration of the product's shelf life, various methods have been employed, among which encapsulation has received much interest. In line with exploitation of encapsulation for protection of probiotics against adverse conditions, we have previously encapsulated bifidobacteria in poly-(vinylpyrrolidone)-poly-(vinylacetate-co-crotonic acid) (PVP:PVAc-CA) interpolymer complex microparticles under supercritical conditions. The microparticles produced had suitable characteristics for food applications and also protected the bacteria in simulated gastrointestinal fluids. The current study reports on accelerated shelf life studies of PVP:PVAc-CA encapsulated Bifidobacterium lactis Bb12 and Bifidobacterium longum Bb46. Samples were stored as free powders in glass vials at 30 °C for 12 weeks and then analysed for viable counts and water activity levels weekly or fortnightly. Water activities of the samples were within the range of 0.25-0.43, with an average a(sub)w = 0.34, throughout the storage period. PVP:PVAc-CA interpolymer complex encapsulation retained viable levels above the recommended minimum for 10 and 12 weeks, for B. longum Bb46 and B. lactis Bb12, respectively, thereby extending their shelf lives under high storage temperature by between 4 and 7 weeks. These results reveal the possibility for manufacture of encapsulated probiotic powders with increased stability at ambient temperatures. This would potentially allow the supply of a stable probiotic formulation to impoverished communities without proper storage facilities recommended for most of the currently available commercial probiotic products.enEncapsulationProbioticsPoly- (vinylpyrrolidone)BifidobacteriaWater activityArticleThantsha, M., Labuschagne, P. W., & Mamvura, C. (2014). Supercritical CO2 interpolymer complex encapsulation improves heat stability of probiotic bifidobacteria. http://hdl.handle.net/10204/7351Thantsha, MS, Philip W Labuschagne, and CI Mamvura "Supercritical CO2 interpolymer complex encapsulation improves heat stability of probiotic bifidobacteria." (2014) http://hdl.handle.net/10204/7351Thantsha M, Labuschagne PW, Mamvura C. Supercritical CO2 interpolymer complex encapsulation improves heat stability of probiotic bifidobacteria. 2014; http://hdl.handle.net/10204/7351.TY - Article AU - Thantsha, MS AU - Labuschagne, Philip W AU - Mamvura, CI AB - The probiotic industry faces the challenge of retention of probiotic culture viability as numbers of these cells within their products inevitably decrease over time. In order to retain probiotic viability levels above the therapeutic minimum over the duration of the product's shelf life, various methods have been employed, among which encapsulation has received much interest. In line with exploitation of encapsulation for protection of probiotics against adverse conditions, we have previously encapsulated bifidobacteria in poly-(vinylpyrrolidone)-poly-(vinylacetate-co-crotonic acid) (PVP:PVAc-CA) interpolymer complex microparticles under supercritical conditions. The microparticles produced had suitable characteristics for food applications and also protected the bacteria in simulated gastrointestinal fluids. The current study reports on accelerated shelf life studies of PVP:PVAc-CA encapsulated Bifidobacterium lactis Bb12 and Bifidobacterium longum Bb46. Samples were stored as free powders in glass vials at 30 °C for 12 weeks and then analysed for viable counts and water activity levels weekly or fortnightly. Water activities of the samples were within the range of 0.25-0.43, with an average a(sub)w = 0.34, throughout the storage period. PVP:PVAc-CA interpolymer complex encapsulation retained viable levels above the recommended minimum for 10 and 12 weeks, for B. longum Bb46 and B. lactis Bb12, respectively, thereby extending their shelf lives under high storage temperature by between 4 and 7 weeks. These results reveal the possibility for manufacture of encapsulated probiotic powders with increased stability at ambient temperatures. This would potentially allow the supply of a stable probiotic formulation to impoverished communities without proper storage facilities recommended for most of the currently available commercial probiotic products. DA - 2014-02 DB - ResearchSpace DP - CSIR KW - Encapsulation KW - Probiotics KW - Poly- (vinylpyrrolidone) KW - Bifidobacteria KW - Water activity LK - https://researchspace.csir.co.za PY - 2014 SM - 0959-3993 T1 - Supercritical CO2 interpolymer complex encapsulation improves heat stability of probiotic bifidobacteria TI - Supercritical CO2 interpolymer complex encapsulation improves heat stability of probiotic bifidobacteria UR - http://hdl.handle.net/10204/7351 ER -