Lalloo, RajeshMoonsamy, GhaneshreeRamchuran, Santosh OGörgens, JGardiner, N2010-03-022010-03-022010Lalloo, R, Moonsamy, G et al. 2010. Competitive exclusion as a mode of action of a novel Bacillus cereus aquaculture biological agent. Letters in Applied Microbiology, Vol.(2010), pp 1-260266-8254http://www3.interscience.wiley.com/journal/123304829/abstracthttp://hdl.handle.net/10204/3963Copyright: 2009 Wiley-Blackwell. This is the author's version of the work. It is posted here by permission of Wiley-Blackwell for your personal use. Not for redistribution. The definitive version was published in the Letters in Applied Microbiology, Vol. (2010)When B. cereus was tested in plate well inhibition studies, no production of antimicrobial compounds was detected. B. cereus had a high growth rate (0.96.h−1), whereas Aer. hydrophila concentration decreased by ∼70% in co-culture experiments. In nutrient limitation studies, B. cereus had a significantly higher growth rate when cultured under glucose (p<0.05) and iron (p<0.01) limitation in comparison to Aer. hydrophila. B. cereus glucose (0.30 g.l−1.h−1) and iron (0.60 mg.l−1.h−1) uptake rates were also significantly higher (p<0.01) than the Aer. hydrophila glucose (0.14 g.l−1.h−1) and iron (0.43 mg.l−1.h−1) uptake rates. Iron uptake was facilitated by siderophore production shown in time profile studies where relative siderophore production was ∼60% through the late exponential and sporulation phases.Competitive exclusion by higher growth rate, competition for organic carbon and iron, facilitated by siderophore production, could be identified as mechanisms of pathogen growth inhibition by B. cereus. This study is the first elucidation of the mechanism of action of our novel B. cereus biological agent in growth attenuation of pathogenic Aer. hydrophila. This study enhances the application knowledge and attractiveness for adoption of B. cereus NRRL 100132 for exploitation in aquaculture.enBacillus sppBiological agentAquacultureSiderophoresMicrobiologyArticleLalloo, R., Moonsamy, G., Ramchuran, S. O., Görgens, J., & Gardiner, N. (2010). Competitive exclusion as a mode of action of a novel Bacillus cereus aquaculture biological agent. http://hdl.handle.net/10204/3963Lalloo, Rajesh, Ghaneshree Moonsamy, Santosh O Ramchuran, J Görgens, and N Gardiner "Competitive exclusion as a mode of action of a novel Bacillus cereus aquaculture biological agent." (2010) http://hdl.handle.net/10204/3963Lalloo R, Moonsamy G, Ramchuran SO, Görgens J, Gardiner N. Competitive exclusion as a mode of action of a novel Bacillus cereus aquaculture biological agent. 2010; http://hdl.handle.net/10204/3963.TY - Article AU - Lalloo, Rajesh AU - Moonsamy, Ghaneshree AU - Ramchuran, Santosh O AU - Görgens, J AU - Gardiner, N AB - When B. cereus was tested in plate well inhibition studies, no production of antimicrobial compounds was detected. B. cereus had a high growth rate (0.96.h−1), whereas Aer. hydrophila concentration decreased by ∼70% in co-culture experiments. In nutrient limitation studies, B. cereus had a significantly higher growth rate when cultured under glucose (p<0.05) and iron (p<0.01) limitation in comparison to Aer. hydrophila. B. cereus glucose (0.30 g.l−1.h−1) and iron (0.60 mg.l−1.h−1) uptake rates were also significantly higher (p<0.01) than the Aer. hydrophila glucose (0.14 g.l−1.h−1) and iron (0.43 mg.l−1.h−1) uptake rates. Iron uptake was facilitated by siderophore production shown in time profile studies where relative siderophore production was ∼60% through the late exponential and sporulation phases.Competitive exclusion by higher growth rate, competition for organic carbon and iron, facilitated by siderophore production, could be identified as mechanisms of pathogen growth inhibition by B. cereus. This study is the first elucidation of the mechanism of action of our novel B. cereus biological agent in growth attenuation of pathogenic Aer. hydrophila. This study enhances the application knowledge and attractiveness for adoption of B. cereus NRRL 100132 for exploitation in aquaculture. DA - 2010 DB - ResearchSpace DP - CSIR KW - Bacillus spp KW - Biological agent KW - Aquaculture KW - Siderophores KW - Microbiology LK - https://researchspace.csir.co.za PY - 2010 SM - 0266-8254 T1 - Competitive exclusion as a mode of action of a novel Bacillus cereus aquaculture biological agent TI - Competitive exclusion as a mode of action of a novel Bacillus cereus aquaculture biological agent UR - http://hdl.handle.net/10204/3963 ER -