Adewusi, EASteenkamp, PFouché, GerdaSteenkamp, V2013-10-232013-10-232013-06Adewusia, E.A, Steenkamp, P, Fouche, G and Steenkamp, V. 2013. Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity. Natural Product Communications, vol. 8(9), pp 1213-12161934-578X1555-9475http://hdl.handle.net/10204/6992Copyright: 2013 Natural Product Communications, vol. 8(9), pp 1213-1216Boophone disticha (Amaryllidaceae) is widely used in traditional medicine in southern Africa. Several alkaloids, volatile oils and fatty acids have been isolated from the plant. However, there has been no literature report of a triterpene from B. disticha. Cycloeucalenol, a cycloartane triterpene, together with its regioisomer, was isolated from the ethyl acetate extract of the bulbs using column chromatography and preparative thin layer chromatography. Structural elucidation was carried out using 1D and 2D NMR and mass spectroscopy. The MTT and neutral red assays were used to assess the cytotoxicity of the compound in human neuroblastoma (SH-SY5Y) cells. The compound was obtained as a mixture of two regio-isomers, which were separated for the first time by chromatographic optimization. Integration of the (sup1)H NMR spectrum showed that cycloeucalenol and its regio-isomer were present in a ratio of 1.04:1. A dose-dependent decrease in cell viability was observed using both cytotoxicity assays. IC(sub50) values of 173.0 ± 5.1 µM and 223.0 ± 6.4 µM were obtained for the MTT and neutral red assays, respectively, indicative of the low toxicity of the compound. This work describes for the first time, the presence of triterpene compounds from the genus Boophone.enAmaryllidaceaeBoophone distichaCycloeucalenolCytotoxicitySH-SY5Y cellsRegio-isomerTraditional plantsArticleAdewusi, E., Steenkamp, P., Fouché, G., & Steenkamp, V. (2013). Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity. http://hdl.handle.net/10204/6992Adewusi, EA, P Steenkamp, Gerda Fouché, and V Steenkamp "Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity." (2013) http://hdl.handle.net/10204/6992Adewusi E, Steenkamp P, Fouché G, Steenkamp V. Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity. 2013; http://hdl.handle.net/10204/6992.TY - Article AU - Adewusi, EA AU - Steenkamp, P AU - Fouché, Gerda AU - Steenkamp, V AB - Boophone disticha (Amaryllidaceae) is widely used in traditional medicine in southern Africa. Several alkaloids, volatile oils and fatty acids have been isolated from the plant. However, there has been no literature report of a triterpene from B. disticha. Cycloeucalenol, a cycloartane triterpene, together with its regioisomer, was isolated from the ethyl acetate extract of the bulbs using column chromatography and preparative thin layer chromatography. Structural elucidation was carried out using 1D and 2D NMR and mass spectroscopy. The MTT and neutral red assays were used to assess the cytotoxicity of the compound in human neuroblastoma (SH-SY5Y) cells. The compound was obtained as a mixture of two regio-isomers, which were separated for the first time by chromatographic optimization. Integration of the (sup1)H NMR spectrum showed that cycloeucalenol and its regio-isomer were present in a ratio of 1.04:1. A dose-dependent decrease in cell viability was observed using both cytotoxicity assays. IC(sub50) values of 173.0 ± 5.1 µM and 223.0 ± 6.4 µM were obtained for the MTT and neutral red assays, respectively, indicative of the low toxicity of the compound. This work describes for the first time, the presence of triterpene compounds from the genus Boophone. DA - 2013-06 DB - ResearchSpace DP - CSIR KW - Amaryllidaceae KW - Boophone disticha KW - Cycloeucalenol KW - Cytotoxicity KW - SH-SY5Y cells KW - Regio-isomer KW - Traditional plants LK - https://researchspace.csir.co.za PY - 2013 SM - 1934-578X SM - 1555-9475 T1 - Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity TI - Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity UR - http://hdl.handle.net/10204/6992 ER -