Visser, Daniel FHennessy, FChhiba, Varsha PLakay, Fvan Heerden, EPiater, LMoller, CLitthauer, DBrady, D2009-01-212009-01-212006-10Visser, DF, Hennessy, F, Chhiba, VP et al. 2006. Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation. University of the Free State, pp 1http://hdl.handle.net/10204/2877Isolating novel enzymes to enhance a commercial process is an area of active research. Biosciences Discovery Biology have established screening methods to isolate genes for novel enzymes from extreme environments. Samples were obtained from four South African mines. Genomic DNA was isolated from these biofilms, and various metagenomic libraries generated. These libraries were in turn screened for industrially important enzymes, in particular proteases and lipases. Resultant hits had plasmid DNA isolated; this DNA was sequenced and analysed using BLASTenDNABioPADAmylaseNitrilaseProteaseLipaseConference PresentationVisser, D. F., Hennessy, F., Chhiba, V. P., Lakay, F., van Heerden, E., Piater, L., ... Brady, D. (2006). Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation. http://hdl.handle.net/10204/2877Visser, Daniel F, F Hennessy, Varsha P Chhiba, F Lakay, E van Heerden, L Piater, C Moller, D Litthauer, and D Brady. "Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation." (2006): http://hdl.handle.net/10204/2877Visser DF, Hennessy F, Chhiba VP, Lakay F, van Heerden E, Piater L, et al, Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation; 2006. http://hdl.handle.net/10204/2877 .TY - Conference Presentation AU - Visser, Daniel F AU - Hennessy, F AU - Chhiba, Varsha P AU - Lakay, F AU - van Heerden, E AU - Piater, L AU - Moller, C AU - Litthauer, D AU - Brady, D AB - Isolating novel enzymes to enhance a commercial process is an area of active research. Biosciences Discovery Biology have established screening methods to isolate genes for novel enzymes from extreme environments. Samples were obtained from four South African mines. Genomic DNA was isolated from these biofilms, and various metagenomic libraries generated. These libraries were in turn screened for industrially important enzymes, in particular proteases and lipases. Resultant hits had plasmid DNA isolated; this DNA was sequenced and analysed using BLAST DA - 2006-10 DB - ResearchSpace DP - CSIR KW - DNA KW - BioPAD KW - Amylase KW - Nitrilase KW - Protease KW - Lipase LK - https://researchspace.csir.co.za PY - 2006 T1 - Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation TI - Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation UR - http://hdl.handle.net/10204/2877 ER -