Louw, MReid, SJJames, DMWatson, TG2007-03-262007-06-072007-03-262007-06-071994-10Louw, M, et al. 1994. Cloning and sequencing the degs-degu operon from an alkalophilic Bacillus-brevis. Applied Microbiology and Biotechnology, vol. 42(1), pp 78-840175-7598http://hdl.handle.net/10204/2022http://hdl.handle.net/10204/2022The sacU region from an alkalophilic Bacillus brevis was cloned and sequenced. The two open reading frames of the degS-degU operon encode polypeptides that gave calculated molecular masses of 43.8 kDa and 27.0 kDa, respectively. Sequence comparisons at the amino acid level to the B. subtilis degS-degU genes showed 74% and 84% similarity, respectively. On a multicopy vector the B. brevis degS-degU genes were found to cause hypersecretion of several extracellular enzymes in a B. subtilis rec (-) strain as well as in a B. subtilis sacU (HY) strain.977645 bytesapplication/pdfenCopyright: 1994 Springer VerlagBacillus brevisDegS-degUSequence comparisonsAmino acidApplied microbiologyArticleLouw, M., Reid, S., James, D., & Watson, T. (1994). Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus-brevis. http://hdl.handle.net/10204/2022Louw, M, SJ Reid, DM James, and TG Watson "Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus-brevis." (1994) http://hdl.handle.net/10204/2022Louw M, Reid S, James D, Watson T. Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus-brevis. 1994; http://hdl.handle.net/10204/2022.TY - Article AU - Louw, M AU - Reid, SJ AU - James, DM AU - Watson, TG AB - The sacU region from an alkalophilic Bacillus brevis was cloned and sequenced. The two open reading frames of the degS-degU operon encode polypeptides that gave calculated molecular masses of 43.8 kDa and 27.0 kDa, respectively. Sequence comparisons at the amino acid level to the B. subtilis degS-degU genes showed 74% and 84% similarity, respectively. On a multicopy vector the B. brevis degS-degU genes were found to cause hypersecretion of several extracellular enzymes in a B. subtilis rec (-) strain as well as in a B. subtilis sacU (HY) strain. DA - 1994-10 DB - ResearchSpace DP - CSIR KW - Bacillus brevis KW - DegS-degU KW - Sequence comparisons KW - Amino acid KW - Applied microbiology LK - https://researchspace.csir.co.za PY - 1994 SM - 0175-7598 T1 - Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus-brevis TI - Cloning and sequencing the degS-degU operon from an alkalophilic Bacillus-brevis UR - http://hdl.handle.net/10204/2022 ER -