Moonsamy, GhaneshreeZulu, Nodumo NokulungaLalloo, RajeshRamchuran, Santosh OSingh, S2020-10-082020-10-082020Moonsamy, G. et al. 2020. Large-scale production of an abalone probiotic, Vibrio midae, isolated from a South African abalone, Halitotis midae for use in aquaculture. Biocatalysis and Agricultural Biotechnology, vol. 29, pp.101878-8181https://www.sciencedirect.com/science/article/pii/S1878818120312585https://doi.org/10.1016/j.bcab.2020.101794https://reader.elsevier.com/reader/sd/pii/S1878818120312585?token=E83D5A75F7362A1F4C5899E797AEC6D96DB0D7EEF56C03C80759D54D4D179F399E50AA76E704AC3F988F0C47A495C439http://hdl.handle.net/10204/11619Copyright: 2020 Elsevier. This is a pre-print version. The definitive version of the work is published in Biocatalysis and Agricultural Biotechnology, vol. 29, pp.10The South African abalone industry is under severe pressure due to illegal harvesting and poaching of this seafood delicacy. These abalone are harvested excessively and consequently, these animals do not have a chance to replace themselves, ensuing in a drastic decrease in natural abalone stocks. Abalone, has an extremely slow growth rate, and takes approximately four years to reach a size that is marketable. Probiotics offer a viable treatment solution to improve the growth, health and vigour of these animals. Two microorganisms, a yeast isolate Debaryomyces hansenii and a bacterial isolate Vibrio midae, from the gut of the South African abalone were characterised for their probiotic abilities. This work resulted in an internationally competitive concept technology that was patented. Thereafter research was focussed on developing a suitable bioprocess to enable commercial production of one the isolates, V. midae. During this development, production parameters such as temperature and pH, as well as alternative medium additives, such as the use of corn steep liquor (CSL) and high test molasses (HTM) was assessed. Once a high efficiency production process was developed at laboratory scale, this up- and downstream production technology was scaled up to manufacturing scale, which resulted in a final market ready probiotic product. The optimization of the key variables resulted in a 180% increase in cell concentration and a 5716-fold increase in cell productivity, in comparison to conventional growth conditions and parameters used at the beginning of the study.enSouth African abalone industryAquacultureProbioticsBioprocessesArticleMoonsamy, G., Zulu, N. N., Lalloo, R., Ramchuran, S. O., & Singh, S. (2020). Large-scale production of an abalone probiotic, Vibrio midae, isolated from a South African abalone, Halitotis midae for use in aquaculture. http://hdl.handle.net/10204/11619Moonsamy, Ghaneshree, Nodumo Nokulunga Zulu, Rajesh Lalloo, Santosh O Ramchuran, and S Singh "Large-scale production of an abalone probiotic, Vibrio midae, isolated from a South African abalone, Halitotis midae for use in aquaculture." (2020) http://hdl.handle.net/10204/11619Moonsamy G, Zulu NN, Lalloo R, Ramchuran SO, Singh S. Large-scale production of an abalone probiotic, Vibrio midae, isolated from a South African abalone, Halitotis midae for use in aquaculture. 2020; http://hdl.handle.net/10204/11619.TY - Article AU - Moonsamy, Ghaneshree AU - Zulu, Nodumo Nokulunga AU - Lalloo, Rajesh AU - Ramchuran, Santosh O AU - Singh, S AB - The South African abalone industry is under severe pressure due to illegal harvesting and poaching of this seafood delicacy. These abalone are harvested excessively and consequently, these animals do not have a chance to replace themselves, ensuing in a drastic decrease in natural abalone stocks. Abalone, has an extremely slow growth rate, and takes approximately four years to reach a size that is marketable. Probiotics offer a viable treatment solution to improve the growth, health and vigour of these animals. Two microorganisms, a yeast isolate Debaryomyces hansenii and a bacterial isolate Vibrio midae, from the gut of the South African abalone were characterised for their probiotic abilities. This work resulted in an internationally competitive concept technology that was patented. Thereafter research was focussed on developing a suitable bioprocess to enable commercial production of one the isolates, V. midae. During this development, production parameters such as temperature and pH, as well as alternative medium additives, such as the use of corn steep liquor (CSL) and high test molasses (HTM) was assessed. Once a high efficiency production process was developed at laboratory scale, this up- and downstream production technology was scaled up to manufacturing scale, which resulted in a final market ready probiotic product. The optimization of the key variables resulted in a 180% increase in cell concentration and a 5716-fold increase in cell productivity, in comparison to conventional growth conditions and parameters used at the beginning of the study. DA - 2020 DB - ResearchSpace DP - CSIR KW - South African abalone industry KW - Aquaculture KW - Probiotics KW - Bioprocesses LK - https://researchspace.csir.co.za PY - 2020 SM - 1878-8181 T1 - Large-scale production of an abalone probiotic, Vibrio midae, isolated from a South African abalone, Halitotis midae for use in aquaculture TI - Large-scale production of an abalone probiotic, Vibrio midae, isolated from a South African abalone, Halitotis midae for use in aquaculture UR - http://hdl.handle.net/10204/11619 ER -