Development of insect cell line using CRISPR technology

Nweke, EEThimiri Govindaraj, Deepak B2021-11-262021-11-262021-02Nweke, E. & Thimiri Govindaraj, D.B. 2021. Development of insect cell line using CRISPR technology. Progress in Molecular Biology and Translational Science, 180. http://hdl.handle.net/10204/121741877-1173https://doi.org/10.1016/bs.pmbts.2021.01.003http://hdl.handle.net/10204/12174In this chapter, we delineated the methods of CRISPR technology that has been used for the development of engineered insect cell line. We elaborated on how CRISPR/Cas9 genome editing in Drosophila melanogaster, Bombyx mori, Spodoptera frugiperda (Sf9 and Sf21), and Mosquitoes enabled the use of model or non-model insect system in various biological and medical applications. Also, the application of synthetic baculovirus genome along with CRISPR/Cas9 vector system to enable genome editing of insect cell systems for treatment of communicable and non-communicable diseases.AbstractenCRISPR/Cas9DrosophilaSpodoptera frugiperdaSf9Sf21Synthetic baculoviral genomeSynBacInsect cell linesDevelopment of insect cell line using CRISPR technologyArticleNweke, E., & Thimiri Govindaraj, D. B. (2021). Development of insect cell line using CRISPR technology. Progress in Molecular Biology and Translational Science, 180, http://hdl.handle.net/10204/12174Nweke, EE, and Deepak B Thimiri Govindaraj "Development of insect cell line using CRISPR technology." Progress in Molecular Biology and Translational Science, 180 (2021) http://hdl.handle.net/10204/12174Nweke E, Thimiri Govindaraj DB. Development of insect cell line using CRISPR technology. Progress in Molecular Biology and Translational Science, 180. 2021; http://hdl.handle.net/10204/12174.TY - Article AU - Nweke, EE AU - Thimiri Govindaraj, Deepak B AB - In this chapter, we delineated the methods of CRISPR technology that has been used for the development of engineered insect cell line. We elaborated on how CRISPR/Cas9 genome editing in Drosophila melanogaster, Bombyx mori, Spodoptera frugiperda (Sf9 and Sf21), and Mosquitoes enabled the use of model or non-model insect system in various biological and medical applications. Also, the application of synthetic baculovirus genome along with CRISPR/Cas9 vector system to enable genome editing of insect cell systems for treatment of communicable and non-communicable diseases. DA - 2021-02 DB - ResearchSpace DP - CSIR J1 - Progress in Molecular Biology and Translational Science, 180 KW - CRISPR/Cas9 KW - Drosophila KW - Spodoptera frugiperda KW - Sf9 KW - Sf21 KW - Synthetic baculoviral genome KW - SynBac KW - Insect cell lines LK - https://researchspace.csir.co.za PY - 2021 SM - 1877-1173 T1 - Development of insect cell line using CRISPR technology TI - Development of insect cell line using CRISPR technology UR - http://hdl.handle.net/10204/12174 ER -25006